Linear fibroblast alignment on sinusoidal wave micropatterns

Jessica R. Gamboa, Samir Mohandes, Phat L. Tran, Marvin J. Slepian, Jeong Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We investigated the behavior of mouse embryonic fibroblasts on non-linear, sinusoidal wave grooves created via electron beam lithography (EBL) on a polymethyl methacrylate (PMMA) substrate. Three different wave patterns, with varying wavelengths and amplitudes, and two linear line patterns were created. For all wave patterns, we noted that cells did not reside within grooves as in line patterns; rather they aligned in a linear fashion, contacting the inside and outside of grooves. 74-81% of cells were aligned on the wave patterns, while 88-95% of cells were aligned on the line patterns.

Original languageEnglish (US)
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1720-1722
Number of pages3
ISBN (Print)9780979806452
StatePublished - Jan 1 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

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Keywords

  • Cell alignment
  • Contact guidance
  • Electron beam lithography
  • Fibroblasts (3T3)
  • Micro patterning
  • Micro topography
  • Polymethyl methacrylate (PMMA)

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Gamboa, J. R., Mohandes, S., Tran, P. L., Slepian, M. J., & Yoon, J. Y. (2012). Linear fibroblast alignment on sinusoidal wave micropatterns. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1720-1722). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.