Linear fibroblast alignment on sinusoidal wave micropatterns

Jessica R. Gamboa, Samir Mohandes, Phat L. Tran, Marvin J Slepian, Jeong-Yeol Yoon

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Micrometer and nanometer grooved surfaces have been determined to influence cellular orientation, morphology, and migration through contact guidance. Cells typically elongate along the direction of an underlying groove and often migrate with guidance provided by constraints of the pattern. This phenomenon has been studied primarily using linear grooves, post, or well patterns. We investigated the behavior of mouse embryonic fibroblasts on non-linear, sinusoidal wave grooves created via electron beam lithography on a polymethyl methacrylate (PMMA) substrate that was spin-coated onto a positively charged glass surface. Three different wave patterns, with varying wavelengths and amplitudes, and two different line patterns were created. Cell orientation and adhesion was examined after 4, 24, and 48. h after cell seeding. Attachment strength was studied via subjecting cells on substrates to centrifugal force following a 24-h incubation period. For all wave patterns studied, it was noted that cells did not reside within the groove, rather they were observed to cross over each groove, residing both inside and outside of each wave pattern, aligning linearly along the long axis of the pattern. For the linear patterns, we observed that cells tended to reside within the grooves, consistent with previous observations. The ability to add texture to a surface to manipulate cell adhesion strength and growth with only localized attachment, maintaining free space in curvilinear microtopography underlying the cell, may be a useful addition for tissue engineering and the fabrication of novel biomedical devices.

Original languageEnglish (US)
Pages (from-to)318-325
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume104
DOIs
StatePublished - Apr 1 2013

Fingerprint

fibroblasts
Fibroblasts
alignment
grooves
cells
Cell Adhesion
Electron beam lithography
Bond strength (materials)
Cell adhesion
Polymethyl Methacrylate
Substrates
Polymethyl methacrylates
Tissue engineering
attachment
adhesion
Adhesion
Textures
Tissue Engineering
Fabrication
Glass

Keywords

  • Cell alignment
  • Contact guidance
  • Electron beam lithography
  • Fibroblasts (3T3)
  • Micro patterning
  • Micro topography

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Linear fibroblast alignment on sinusoidal wave micropatterns. / Gamboa, Jessica R.; Mohandes, Samir; Tran, Phat L.; Slepian, Marvin J; Yoon, Jeong-Yeol.

In: Colloids and Surfaces B: Biointerfaces, Vol. 104, 01.04.2013, p. 318-325.

Research output: Contribution to journalArticle

Gamboa, Jessica R. ; Mohandes, Samir ; Tran, Phat L. ; Slepian, Marvin J ; Yoon, Jeong-Yeol. / Linear fibroblast alignment on sinusoidal wave micropatterns. In: Colloids and Surfaces B: Biointerfaces. 2013 ; Vol. 104. pp. 318-325.
@article{41c8923e70d84c038af0e5fd7b257a25,
title = "Linear fibroblast alignment on sinusoidal wave micropatterns",
abstract = "Micrometer and nanometer grooved surfaces have been determined to influence cellular orientation, morphology, and migration through contact guidance. Cells typically elongate along the direction of an underlying groove and often migrate with guidance provided by constraints of the pattern. This phenomenon has been studied primarily using linear grooves, post, or well patterns. We investigated the behavior of mouse embryonic fibroblasts on non-linear, sinusoidal wave grooves created via electron beam lithography on a polymethyl methacrylate (PMMA) substrate that was spin-coated onto a positively charged glass surface. Three different wave patterns, with varying wavelengths and amplitudes, and two different line patterns were created. Cell orientation and adhesion was examined after 4, 24, and 48. h after cell seeding. Attachment strength was studied via subjecting cells on substrates to centrifugal force following a 24-h incubation period. For all wave patterns studied, it was noted that cells did not reside within the groove, rather they were observed to cross over each groove, residing both inside and outside of each wave pattern, aligning linearly along the long axis of the pattern. For the linear patterns, we observed that cells tended to reside within the grooves, consistent with previous observations. The ability to add texture to a surface to manipulate cell adhesion strength and growth with only localized attachment, maintaining free space in curvilinear microtopography underlying the cell, may be a useful addition for tissue engineering and the fabrication of novel biomedical devices.",
keywords = "Cell alignment, Contact guidance, Electron beam lithography, Fibroblasts (3T3), Micro patterning, Micro topography",
author = "Gamboa, {Jessica R.} and Samir Mohandes and Tran, {Phat L.} and Slepian, {Marvin J} and Jeong-Yeol Yoon",
year = "2013",
month = "4",
day = "1",
doi = "10.1016/j.colsurfb.2012.11.035",
language = "English (US)",
volume = "104",
pages = "318--325",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

}

TY - JOUR

T1 - Linear fibroblast alignment on sinusoidal wave micropatterns

AU - Gamboa, Jessica R.

AU - Mohandes, Samir

AU - Tran, Phat L.

AU - Slepian, Marvin J

AU - Yoon, Jeong-Yeol

PY - 2013/4/1

Y1 - 2013/4/1

N2 - Micrometer and nanometer grooved surfaces have been determined to influence cellular orientation, morphology, and migration through contact guidance. Cells typically elongate along the direction of an underlying groove and often migrate with guidance provided by constraints of the pattern. This phenomenon has been studied primarily using linear grooves, post, or well patterns. We investigated the behavior of mouse embryonic fibroblasts on non-linear, sinusoidal wave grooves created via electron beam lithography on a polymethyl methacrylate (PMMA) substrate that was spin-coated onto a positively charged glass surface. Three different wave patterns, with varying wavelengths and amplitudes, and two different line patterns were created. Cell orientation and adhesion was examined after 4, 24, and 48. h after cell seeding. Attachment strength was studied via subjecting cells on substrates to centrifugal force following a 24-h incubation period. For all wave patterns studied, it was noted that cells did not reside within the groove, rather they were observed to cross over each groove, residing both inside and outside of each wave pattern, aligning linearly along the long axis of the pattern. For the linear patterns, we observed that cells tended to reside within the grooves, consistent with previous observations. The ability to add texture to a surface to manipulate cell adhesion strength and growth with only localized attachment, maintaining free space in curvilinear microtopography underlying the cell, may be a useful addition for tissue engineering and the fabrication of novel biomedical devices.

AB - Micrometer and nanometer grooved surfaces have been determined to influence cellular orientation, morphology, and migration through contact guidance. Cells typically elongate along the direction of an underlying groove and often migrate with guidance provided by constraints of the pattern. This phenomenon has been studied primarily using linear grooves, post, or well patterns. We investigated the behavior of mouse embryonic fibroblasts on non-linear, sinusoidal wave grooves created via electron beam lithography on a polymethyl methacrylate (PMMA) substrate that was spin-coated onto a positively charged glass surface. Three different wave patterns, with varying wavelengths and amplitudes, and two different line patterns were created. Cell orientation and adhesion was examined after 4, 24, and 48. h after cell seeding. Attachment strength was studied via subjecting cells on substrates to centrifugal force following a 24-h incubation period. For all wave patterns studied, it was noted that cells did not reside within the groove, rather they were observed to cross over each groove, residing both inside and outside of each wave pattern, aligning linearly along the long axis of the pattern. For the linear patterns, we observed that cells tended to reside within the grooves, consistent with previous observations. The ability to add texture to a surface to manipulate cell adhesion strength and growth with only localized attachment, maintaining free space in curvilinear microtopography underlying the cell, may be a useful addition for tissue engineering and the fabrication of novel biomedical devices.

KW - Cell alignment

KW - Contact guidance

KW - Electron beam lithography

KW - Fibroblasts (3T3)

KW - Micro patterning

KW - Micro topography

UR - http://www.scopus.com/inward/record.url?scp=84874379314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874379314&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2012.11.035

DO - 10.1016/j.colsurfb.2012.11.035

M3 - Article

C2 - 23375052

AN - SCOPUS:84874379314

VL - 104

SP - 318

EP - 325

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -