Linking gene expression to mechanisms of toxicity

James L. Stevens, Hong Liu, Margaretann Halleck, Russell C. Bowes, Qin Chen, Bob Van De Water

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Activation of gene expression is one of the earliest cellular responses to toxicity. However, our understanding of the biological and biochemical signals that activate these toxicant-responsive genes as well as the consequences of gene activation to survival of the organism remains sketchy. In this article, strategies that can be used to link changes in gene expression to biochemical mechanisms of toxicity are addressed using the hsp70 and grp78 genes as examples. The data indicate that activation of hsp70 is linked to changes in thiol-disulfide redox perturbations while grp78 activation may be caused by loss of calcium from the endoplasmic reticulum. Each gene is part of a discrete feedback regulated signaling pathway designed to protect cells against the toxic signals that activate gene expression. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)479-486
Number of pages8
JournalToxicology Letters
Volume112-113
DOIs
StatePublished - Mar 15 2000

Fingerprint

Gene expression
Toxicity
Genes
Chemical activation
Gene Expression
Poisons
Sulfhydryl Compounds
Endoplasmic Reticulum
Disulfides
Transcriptional Activation
Oxidation-Reduction
Calcium
Feedback

Keywords

  • Calcium
  • Cellular toxicity
  • Gene expression
  • Glutathione
  • hsp70
  • Lipid peroxidation
  • Reactive oxygen species

ASJC Scopus subject areas

  • Toxicology

Cite this

Stevens, J. L., Liu, H., Halleck, M., Bowes, R. C., Chen, Q., & Van De Water, B. (2000). Linking gene expression to mechanisms of toxicity. Toxicology Letters, 112-113, 479-486. https://doi.org/10.1016/S0378-4274(99)00200-3

Linking gene expression to mechanisms of toxicity. / Stevens, James L.; Liu, Hong; Halleck, Margaretann; Bowes, Russell C.; Chen, Qin; Van De Water, Bob.

In: Toxicology Letters, Vol. 112-113, 15.03.2000, p. 479-486.

Research output: Contribution to journalArticle

Stevens, JL, Liu, H, Halleck, M, Bowes, RC, Chen, Q & Van De Water, B 2000, 'Linking gene expression to mechanisms of toxicity', Toxicology Letters, vol. 112-113, pp. 479-486. https://doi.org/10.1016/S0378-4274(99)00200-3
Stevens JL, Liu H, Halleck M, Bowes RC, Chen Q, Van De Water B. Linking gene expression to mechanisms of toxicity. Toxicology Letters. 2000 Mar 15;112-113:479-486. https://doi.org/10.1016/S0378-4274(99)00200-3
Stevens, James L. ; Liu, Hong ; Halleck, Margaretann ; Bowes, Russell C. ; Chen, Qin ; Van De Water, Bob. / Linking gene expression to mechanisms of toxicity. In: Toxicology Letters. 2000 ; Vol. 112-113. pp. 479-486.
@article{c6de418d976b48989c12d204ab7f3b62,
title = "Linking gene expression to mechanisms of toxicity",
abstract = "Activation of gene expression is one of the earliest cellular responses to toxicity. However, our understanding of the biological and biochemical signals that activate these toxicant-responsive genes as well as the consequences of gene activation to survival of the organism remains sketchy. In this article, strategies that can be used to link changes in gene expression to biochemical mechanisms of toxicity are addressed using the hsp70 and grp78 genes as examples. The data indicate that activation of hsp70 is linked to changes in thiol-disulfide redox perturbations while grp78 activation may be caused by loss of calcium from the endoplasmic reticulum. Each gene is part of a discrete feedback regulated signaling pathway designed to protect cells against the toxic signals that activate gene expression. Copyright (C) 2000 Elsevier Science Ireland Ltd.",
keywords = "Calcium, Cellular toxicity, Gene expression, Glutathione, hsp70, Lipid peroxidation, Reactive oxygen species",
author = "Stevens, {James L.} and Hong Liu and Margaretann Halleck and Bowes, {Russell C.} and Qin Chen and {Van De Water}, Bob",
year = "2000",
month = "3",
day = "15",
doi = "10.1016/S0378-4274(99)00200-3",
language = "English (US)",
volume = "112-113",
pages = "479--486",
journal = "Toxicology Letters",
issn = "0378-4274",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Linking gene expression to mechanisms of toxicity

AU - Stevens, James L.

AU - Liu, Hong

AU - Halleck, Margaretann

AU - Bowes, Russell C.

AU - Chen, Qin

AU - Van De Water, Bob

PY - 2000/3/15

Y1 - 2000/3/15

N2 - Activation of gene expression is one of the earliest cellular responses to toxicity. However, our understanding of the biological and biochemical signals that activate these toxicant-responsive genes as well as the consequences of gene activation to survival of the organism remains sketchy. In this article, strategies that can be used to link changes in gene expression to biochemical mechanisms of toxicity are addressed using the hsp70 and grp78 genes as examples. The data indicate that activation of hsp70 is linked to changes in thiol-disulfide redox perturbations while grp78 activation may be caused by loss of calcium from the endoplasmic reticulum. Each gene is part of a discrete feedback regulated signaling pathway designed to protect cells against the toxic signals that activate gene expression. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - Activation of gene expression is one of the earliest cellular responses to toxicity. However, our understanding of the biological and biochemical signals that activate these toxicant-responsive genes as well as the consequences of gene activation to survival of the organism remains sketchy. In this article, strategies that can be used to link changes in gene expression to biochemical mechanisms of toxicity are addressed using the hsp70 and grp78 genes as examples. The data indicate that activation of hsp70 is linked to changes in thiol-disulfide redox perturbations while grp78 activation may be caused by loss of calcium from the endoplasmic reticulum. Each gene is part of a discrete feedback regulated signaling pathway designed to protect cells against the toxic signals that activate gene expression. Copyright (C) 2000 Elsevier Science Ireland Ltd.

KW - Calcium

KW - Cellular toxicity

KW - Gene expression

KW - Glutathione

KW - hsp70

KW - Lipid peroxidation

KW - Reactive oxygen species

UR - http://www.scopus.com/inward/record.url?scp=0034653363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034653363&partnerID=8YFLogxK

U2 - 10.1016/S0378-4274(99)00200-3

DO - 10.1016/S0378-4274(99)00200-3

M3 - Article

C2 - 10720769

AN - SCOPUS:0034653363

VL - 112-113

SP - 479

EP - 486

JO - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

ER -