Linking gene expression to mechanisms of toxicity

James L. Stevens, Hong Liu, Margaretann Halleck, Russell C. Bowes, Qin Mary Chen, Bob Van De Water

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Activation of gene expression is one of the earliest cellular responses to toxicity. However, our understanding of the biological and biochemical signals that activate these toxicant-responsive genes as well as the consequences of gene activation to survival of the organism remains sketchy. In this article, strategies that can be used to link changes in gene expression to biochemical mechanisms of toxicity are addressed using the hsp70 and grp78 genes as examples. The data indicate that activation of hsp70 is linked to changes in thiol-disulfide redox perturbations while grp78 activation may be caused by loss of calcium from the endoplasmic reticulum. Each gene is part of a discrete feedback regulated signaling pathway designed to protect cells against the toxic signals that activate gene expression. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)479-486
Number of pages8
JournalToxicology letters
Volume112-113
DOIs
StatePublished - Mar 15 2000

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Keywords

  • Calcium
  • Cellular toxicity
  • Gene expression
  • Glutathione
  • Lipid peroxidation
  • Reactive oxygen species
  • hsp70

ASJC Scopus subject areas

  • Toxicology

Cite this

Stevens, J. L., Liu, H., Halleck, M., Bowes, R. C., Chen, Q. M., & Van De Water, B. (2000). Linking gene expression to mechanisms of toxicity. Toxicology letters, 112-113, 479-486. https://doi.org/10.1016/S0378-4274(99)00200-3