Lipid bilayer dynamics and rhodopsin-lipid interactions: New approach using high-resolution solid-state 13C NMR

Michael D. Sefcik, Jacob Schaefer, Edward O. Stejskal, Robert A. McKay, Jeffrey F. Ellena, Steven W. Dodd, Michael F. Brown

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

High-resolution, solid-state 13C NMR spectra have been obtained for unsonicated multilamellar dispersions of 1,2-dilauryl-sn-glycero-3-phosphocholine (DLPC), recombinant membranes containing DLPC and rhodopsin, and native retinal rod disk membranes. The roles of 1H dipolar decoupling, 1H-13C cross-polarization, and magic-angle sample spinning have been investigated. Rotating-frame 13C relaxation times have been measured and are discussed in terms of lipid bilayer dynamics and rhodopsin-lipid interactions.

Original languageEnglish (US)
Pages (from-to)1048-1055
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume114
Issue number3
DOIs
StatePublished - Aug 12 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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