Lipid detection, identification, and imaging single cells with SIMS

Michael L. Heien, Paul D. Piehowski, Nicholas Winograd, Andrew G. Ewing

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) can be utilized to map the distribution of various molecules on a surface with submicrometer resolution. Many of its biological applications have been in the study of membrane lipids, such as phospholipids and cholesterol. For these studies, the effectiveness of chemical mapping is limited by low signal intensity from various biomolecules. Because of the high-energy nature of the SIMS ionization process, many molecules are identified by detection of characteristic fragments. Cluster ion sources are able to increase ionization, leading to increased information collected from a surface. In this chapter, we highlight the utility of SIMS to image lipids at single cells. Particularly, we will describe sample preparation, data collection, and the analysis of lipids for two systems; rat oligodendrocytes and Tetrahymena thermophila. SIMS spectra yield information regarding lipid identity and concentration across cell surface.

Original languageEnglish (US)
Title of host publicationMass Spectrometry Imaging
Subtitle of host publicationPrinciples and Protocols
EditorsStanislav Rubakhin, Jonathan Sweedler
Pages85-97
Number of pages13
DOIs
StatePublished - Dec 1 2010

Publication series

NameMethods in Molecular Biology
Volume656
ISSN (Print)1064-3745

Keywords

  • Time-of-flight (ToF)
  • freeze-etch
  • freeze-fracture
  • imaging
  • lipids
  • mass spectrometry
  • secondary ion mass spectrometry (SIMS)
  • single cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Heien, M. L., Piehowski, P. D., Winograd, N., & Ewing, A. G. (2010). Lipid detection, identification, and imaging single cells with SIMS. In S. Rubakhin, & J. Sweedler (Eds.), Mass Spectrometry Imaging: Principles and Protocols (pp. 85-97). (Methods in Molecular Biology; Vol. 656). https://doi.org/10.1007/978-1-60761-746-4_4