Lipid-mediated protein functionalization of electrospun polycaprolactone fibers

C. Cohn, S. L. Leung, J. Crosby, B. Lafuente, Z. Zha, W. Teng, Robert T Downs, Xiaoyi Wu

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

In this study, electrospun polycaprolactone (PCL) fibers are plasma-treated and chemically conjugated with cholesteryl succinyl silane (CSS). In addition to Raman spectroscopy, an immobilization study of DiO as a fluorescent probe of lipid membranes provides evidence supporting the CSS coating of plasma-treated PCL fibers. Further, anti-CD20 antibodies are used as a model protein to evaluate the potential of lipid-mediated protein immobilization as a mechanism to functionalize the CSS-PCL fiber scaffolds. Upon anti-CD20 functionalization, the CSS-PCL fiber scaffolds capture Granta-22 cells 2.4 times more than the PCL control does, although the two fiber scaffolds immobilize a comparable amount of anti- CD20. Taken together, results from the present study demonstrate that the CSS coating and CSS-mediated antibody immobilization offers an appealing strategy to functionalize electrospun synthetic polymer fibers and confer cell-specific functions on the fiber scaffolds, which can be mechanically robust but often lack biological functions.

Original languageEnglish (US)
Pages (from-to)430-437
Number of pages8
JournalExpress Polymer Letters
Volume10
Issue number5
DOIs
StatePublished - May 1 2016

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Keywords

  • Cholesteryl succinyl silane
  • Coatings
  • Electrospun polycaprolactone fibers
  • Protein immobilization

ASJC Scopus subject areas

  • Polymers and Plastics
  • Materials Chemistry
  • Chemical Engineering(all)
  • Organic Chemistry
  • Physical and Theoretical Chemistry

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