Lipid-protein interactions mediate the photochemical function of rhodopsin

Timothy S. Wiedmann, Robert D. Pates, James M. Beach, Amir Salmon, Michael F Brown

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

We have investigated the molecular features of recombinant membranes that are necessary for the photochemical function of rhodopsin. The magnitude of the metarhodopsin I to metarhodopsin II phototransient following a 25% ± 3% bleaching flash was used as a criterion of photochemical activity at 28°C and pH 7.0. Nativelike activity of rhodopsin can be reconstituted with an extract of total lipids from rod outer segment membranes, demonstrating that the protein is minimally perturbed by the reconstitution protocol. Rhodopsin photochemical activity is enhanced by phosphatidylethanolamine head groups and docosahexaenoyl (22:6ω3) acyl chains. An equimolar mixture of phosphatidylethanolamine and phosphatidylcholine containing 50 mol % docosahexaenoyl chains results in optimal photochemical function. These results suggest the importance of both the head-group and acyl chain composition of the rod outer segment lipids in the visual process. The extracted rod lipids and those lipid mixtures favoring the conformational change from metarhodopsin I to II can undergo lamellar (Lα) to inverted hexagonal (HII) phase transitions near physiological temperature. Interaction of rhodopsin with membrane lipids close to a Lα to HII (or cubic) phase boundary may thus lead to properties which influence the energetics of conformational states of the protein linked to visual function.

Original languageEnglish (US)
Pages (from-to)6469-6474
Number of pages6
JournalBiochemistry
Volume27
Issue number17
StatePublished - 1988

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Rhodopsin
Rod Cell Outer Segment
Lipids
Proteins
Phase Transition
Phase boundaries
Membrane Lipids
Bleaching
Phosphatidylcholines
Membrane Proteins
Phase transitions
Head
Membranes
Temperature
Chemical analysis
metarhodopsins
phosphatidylethanolamine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Wiedmann, T. S., Pates, R. D., Beach, J. M., Salmon, A., & Brown, M. F. (1988). Lipid-protein interactions mediate the photochemical function of rhodopsin. Biochemistry, 27(17), 6469-6474.

Lipid-protein interactions mediate the photochemical function of rhodopsin. / Wiedmann, Timothy S.; Pates, Robert D.; Beach, James M.; Salmon, Amir; Brown, Michael F.

In: Biochemistry, Vol. 27, No. 17, 1988, p. 6469-6474.

Research output: Contribution to journalArticle

Wiedmann, TS, Pates, RD, Beach, JM, Salmon, A & Brown, MF 1988, 'Lipid-protein interactions mediate the photochemical function of rhodopsin', Biochemistry, vol. 27, no. 17, pp. 6469-6474.
Wiedmann TS, Pates RD, Beach JM, Salmon A, Brown MF. Lipid-protein interactions mediate the photochemical function of rhodopsin. Biochemistry. 1988;27(17):6469-6474.
Wiedmann, Timothy S. ; Pates, Robert D. ; Beach, James M. ; Salmon, Amir ; Brown, Michael F. / Lipid-protein interactions mediate the photochemical function of rhodopsin. In: Biochemistry. 1988 ; Vol. 27, No. 17. pp. 6469-6474.
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