Liquid-chromatographic determination of ciprofloxacin in serum and urine

David E. Nix, J. M. De Vito, J. J. Schentag

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

We describe the liquid-chromatographic determination of ciprofloxacin in patients' serum and urine. Serum samples were prepared by precipitating protein with perchloric acid. Urine samples were diluted 100-fold with mobile phase. The mobile phase, consisting of pH 3 phosphate buffer/acetonitrile/methanol (81/5/14, by vol), was pumped through a μBondapak C18 reversed-phase column at 1.5 mL/min. Fluorescence of the effluent was monitored, at wavelengths for excitation and emission of 270 and 440 nm, respectively. Standard curves were linearly related to concentration from 0.08 to 10 mg/L for serum, 1 to 20 mg/L for urine. The procedure was evaluated in a clinical setting to determine its usefulness in studying the pharmacokinetics of ciprofloxacin in patients with concurrent diseases and receiving multiple drug therapies.

Original languageEnglish (US)
Pages (from-to)684-686
Number of pages3
JournalClinical Chemistry
Volume31
Issue number5
StatePublished - 1985
Externally publishedYes

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Ciprofloxacin
Urine
Drug therapy
Pharmacokinetics
Liquids
Serum
Methanol
Effluents
Buffers
Fluorescence
Phosphates
Wavelength
Drug Therapy
Proteins
Perchloric Acid
acetonitrile

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Liquid-chromatographic determination of ciprofloxacin in serum and urine. / Nix, David E.; De Vito, J. M.; Schentag, J. J.

In: Clinical Chemistry, Vol. 31, No. 5, 1985, p. 684-686.

Research output: Contribution to journalArticle

Nix, DE, De Vito, JM & Schentag, JJ 1985, 'Liquid-chromatographic determination of ciprofloxacin in serum and urine', Clinical Chemistry, vol. 31, no. 5, pp. 684-686.
Nix, David E. ; De Vito, J. M. ; Schentag, J. J. / Liquid-chromatographic determination of ciprofloxacin in serum and urine. In: Clinical Chemistry. 1985 ; Vol. 31, No. 5. pp. 684-686.
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