Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration

Erin Greenwood, Sabrina Maisel, David Ebertz, Atlantis Russ, Ritu Pandey, Joyce Schroeder

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We have previously demonstrated that Llgl1 loss results in a gain of mesenchymal phenotypes and a loss of apicobasal and planar polarity. We now demonstrate that these changes represent a fundamental shift in cellular phenotype. Llgl1 regulates the expression of multiple cell identity markers, including CD44, CD49f, and CD24, and the nuclear translocation of TAZ and Slug. Cells lacking Llgl1 form mammospheres, where survival and transplantability is dependent upon the Epidermal Growth Factor Receptor (EGFR). Additionally, Llgl1 loss allows cells to grow in soft-agar and maintain prolonged survival as orthotopic transplants in NOD-SCID mice. Lineage tracing and wound healing experiments demonstrate that mammosphere survival is due to enhanced EGF-dependent migration. The loss of Llgl1 drives EGFR mislocalization and an EGFR mislocalization point mutation (P667A) drives these same phenotypes, including activation of AKT and TAZ nuclear translocation. Together, these data indicate that the loss of Llgl1 results in EGFR mislocalization, promoting pre-neoplastic changes.

Original languageEnglish (US)
Pages (from-to)60776-60792
Number of pages17
JournalOncotarget
Volume7
Issue number38
DOIs
StatePublished - 2016

Keywords

  • Epidermal growth factor receptor
  • Llgl1
  • Migration
  • Polarity
  • TAZ

ASJC Scopus subject areas

  • Oncology

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