Abstract
The effects on the in vitro lymphocyte blastogenic response to phytohemagglutinin and streptolysin O were studied in 10 patients. All patients had melanoma of the extremities and were treated with a combination of phenylalanine mustard, actinomycin D, and nitrogen mustard. Profound local effects of the perfusion were noted. Lymphocyte blastogenic responses of cells collected during therapy were markedly impaired. This was true whether the cells were cultured in patient serum or normal serum. In general, at least statistically, no systemic effects of the perfusion therapy were noted. However, in several of the 10 patients there was a significant decline in the postperfusion lymphocyte blastogenic responses, even in normal serum. This suggests that either stress of the procedure or leakage of the perfused drug suppressed blastogenic responses of the general circulating lymphocytes. There was no correlation of the suppressed responses after perfusion with the degree of leakage and, therefore, stress seems a more reasonable explanation. In 6 persons, the preperfusion lymphocyte responses were significantly higher in normal serum. These patients who had only localized disease had a serum inhibitory factor which previously has been noted only in patients with metastatic disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 461-464 |
| Number of pages | 4 |
| Journal | Surgery Gynecology and Obstetrics |
| Volume | 137 |
| Issue number | 3 |
| State | Published - 1973 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Obstetrics and Gynecology
- Surgery
Cite this
Local and systemic immunologic effects of perfusion therapy for malignant melanoma. / Hersh, Evan M; McBride, C. M.; Gschwind, C.
In: Surgery Gynecology and Obstetrics, Vol. 137, No. 3, 1973, p. 461-464.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Local and systemic immunologic effects of perfusion therapy for malignant melanoma
AU - Hersh, Evan M
AU - McBride, C. M.
AU - Gschwind, C.
PY - 1973
Y1 - 1973
N2 - The effects on the in vitro lymphocyte blastogenic response to phytohemagglutinin and streptolysin O were studied in 10 patients. All patients had melanoma of the extremities and were treated with a combination of phenylalanine mustard, actinomycin D, and nitrogen mustard. Profound local effects of the perfusion were noted. Lymphocyte blastogenic responses of cells collected during therapy were markedly impaired. This was true whether the cells were cultured in patient serum or normal serum. In general, at least statistically, no systemic effects of the perfusion therapy were noted. However, in several of the 10 patients there was a significant decline in the postperfusion lymphocyte blastogenic responses, even in normal serum. This suggests that either stress of the procedure or leakage of the perfused drug suppressed blastogenic responses of the general circulating lymphocytes. There was no correlation of the suppressed responses after perfusion with the degree of leakage and, therefore, stress seems a more reasonable explanation. In 6 persons, the preperfusion lymphocyte responses were significantly higher in normal serum. These patients who had only localized disease had a serum inhibitory factor which previously has been noted only in patients with metastatic disease.
AB - The effects on the in vitro lymphocyte blastogenic response to phytohemagglutinin and streptolysin O were studied in 10 patients. All patients had melanoma of the extremities and were treated with a combination of phenylalanine mustard, actinomycin D, and nitrogen mustard. Profound local effects of the perfusion were noted. Lymphocyte blastogenic responses of cells collected during therapy were markedly impaired. This was true whether the cells were cultured in patient serum or normal serum. In general, at least statistically, no systemic effects of the perfusion therapy were noted. However, in several of the 10 patients there was a significant decline in the postperfusion lymphocyte blastogenic responses, even in normal serum. This suggests that either stress of the procedure or leakage of the perfused drug suppressed blastogenic responses of the general circulating lymphocytes. There was no correlation of the suppressed responses after perfusion with the degree of leakage and, therefore, stress seems a more reasonable explanation. In 6 persons, the preperfusion lymphocyte responses were significantly higher in normal serum. These patients who had only localized disease had a serum inhibitory factor which previously has been noted only in patients with metastatic disease.
UR - http://www.scopus.com/inward/record.url?scp=0015781870&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015781870&partnerID=8YFLogxK
M3 - Article
C2 - 4721519
AN - SCOPUS:0015781870
VL - 137
SP - 461
EP - 464
JO - Journal of the American College of Surgeons
JF - Journal of the American College of Surgeons
SN - 1072-7515
IS - 3
ER -