Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects

Florian Grüner, Florian Blumendorf, Oliver Schmutzler, Theresa Staufer, Michelle Bradbury, Ulrich Wiesner, Tanja Rosentreter, Gabriele Loers, David Lutz, Bernadette Richter, Markus Fischer, Florian Schulz, Swantje Steiner, Martin Warmer, Anja Burkhardt, Alke Meents, Matthew Kupinski, Christoph Hoeschen

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Accurate in vivo localisation of minimal amounts of functionalised gold-nanoparticles, enabling e.g. early-tumour diagnostics and pharmacokinetic tracking studies, requires a precision imaging system offering very high sensitivity, temporal and spatial resolution, large depth penetration, and arbitrarily long serial measurements. X-ray fluorescence imaging could offer such capabilities; however, its utilisation for human-sized scales is hampered by a high intrinsic background level. Here we measure and model this anisotropic background and present a spatial filtering scheme for background reduction enabling the localisation of nanoparticle-amounts as reported from small-animal tumour models. As a basic application study towards precision pharmacokinetics, we demonstrate specific localisation to sites of disease by adapting gold-nanoparticles with small targeting ligands in murine spinal cord injury models, at record sensitivity levels using sub-mm resolution. Both studies contribute to the future use of molecularly-targeted gold-nanoparticles as next-generation clinical diagnostic and pharmacokinetic tools.

Original languageEnglish (US)
Article number16561
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

ASJC Scopus subject areas

  • General

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    Grüner, F., Blumendorf, F., Schmutzler, O., Staufer, T., Bradbury, M., Wiesner, U., Rosentreter, T., Loers, G., Lutz, D., Richter, B., Fischer, M., Schulz, F., Steiner, S., Warmer, M., Burkhardt, A., Meents, A., Kupinski, M., & Hoeschen, C. (2018). Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects. Scientific reports, 8(1), [16561]. https://doi.org/10.1038/s41598-018-34925-3