Localization and chemical characterization of a partially purified bovine pineal antigonadotropin

I. Y. Rosenblum, B. Benson, C. F. Bria, D. McDonnell, Victor J Hruby

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An antigonadotropic substance was partially purified from aqueous extracts of bovine pineal glands by methods of gel filtration, ultrafiltration and ion exchange chromatography. Two biological tests, viz. inhibition of compensatory ovarian hypertrophy and reduction of ventral prostate weight, were used to guide the purification. The partially purified antigonadotropin was characterized chemically using techniques of UV and fluorescence spectrometry, thin layer and paper chromatography, paper electrophoresis and amino acid analysis. The results reveal a diversity of ninhydrin-positive components present in the preparations, including free and peptide-bound amino acids, as well as other unidentified components, but not including any of the commonly occurring indoles, indoleamines or catecholeamines. One peptide, oxidized glutathione, was identified in the most purified material containing the biologically active principle yet pure, synthetric glutathione has no antigonadotropic activity in the biological tests utilized. Although the chemical nature of the bovine pineal antigonadotropin remains in question it may be purified by the methods described. The activity is thought to reside in the extremely small, perhaps trace quantities of residues derived. It is believed that large scale, preparative studies will be required for structural determination.

Original languageEnglish (US)
Pages (from-to)197-220
Number of pages24
JournalJournal of Neural Transmission
Volume44
Issue number3
DOIs
StatePublished - Sep 1979

Fingerprint

Ninhydrin
Paper Electrophoresis
Indoles
Amino Acids
Paper Chromatography
Peptides
Pineal Gland
Glutathione Disulfide
Fluorescence Spectrometry
Ion Exchange Chromatography
Ultrafiltration
Thin Layer Chromatography
Hypertrophy
Gel Chromatography
Glutathione
Prostate
Weights and Measures
pineal antigonadotropin

Keywords

  • bovine pineal extracts
  • Pineal
  • pineal antigonadotropin
  • pineal peptides

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Localization and chemical characterization of a partially purified bovine pineal antigonadotropin. / Rosenblum, I. Y.; Benson, B.; Bria, C. F.; McDonnell, D.; Hruby, Victor J.

In: Journal of Neural Transmission, Vol. 44, No. 3, 09.1979, p. 197-220.

Research output: Contribution to journalArticle

Rosenblum, I. Y. ; Benson, B. ; Bria, C. F. ; McDonnell, D. ; Hruby, Victor J. / Localization and chemical characterization of a partially purified bovine pineal antigonadotropin. In: Journal of Neural Transmission. 1979 ; Vol. 44, No. 3. pp. 197-220.
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