Localization of dipeptidyl peptidase-4 (CD26) to human pancreatic ducts and islet alpha cells

Petra Augstein, Gaetano Naselli, Thomas Loudovaris, Wayne J. Hawthorne, Peter Campbell, Esther Bandala-Sanchez, Kelly Rogers, Peter Heinke, Helen E. Thomas, Thomas W. Kay, Leonard C. Harrison

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Aim: DPP-4/CD26 degrades the incretins GLP-1 and GIP. The localization of DPP-4 within the human pancreas is not well documented but is likely to be relevant for understanding incretin function. We aimed to define the cellular localization of DPP-4 in the human pancreas from cadaveric organ donors with and without diabetes. Methods: Pancreas was snap-frozen and immunoreactive DPP-4 detected in cryosections using the APAAP technique. For co-localization studies, pancreas sections were double-stained for DPP-4 and proinsulin or glucagon and scanned by confocal microscopy. Pancreata were digested and cells in islets and in islet-depleted, duct-enriched digests analyzed for expression of DPP-4 and other markers by flow cytometry. Results: DPP-4 was expressed by pancreatic duct and islet cells. In pancreata from donors without diabetes or with type 2 diabetes, DPP-4-positive cells in islets had the same location and morphology as glucagon-positive cells, and the expression of DPP-4 and glucagon overlapped. In donors with type 1 diabetes, the majority of residual cells in islets were DPP-4-positive. Conclusion: In the human pancreas, DPP-4 expression is localized to duct and alpha cells. This finding is consistent with the view that DPP-4 regulates exposure to incretins of duct cells directly and of beta cells indirectly in a paracrine manner.

Original languageEnglish (US)
Pages (from-to)291-300
Number of pages10
JournalDiabetes Research and Clinical Practice
Volume110
Issue number3
DOIs
StatePublished - Dec 1 2015
Externally publishedYes

Keywords

  • Alpha cell
  • Beta cell
  • CD26
  • Dipeptidyl peptidase-4
  • Human pancreas
  • Islet

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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