Localization of ghrelin and its receptor in the reproductive tract of Holstein heifers

S. E. Deaver, Patricia B Hoyer, S. M. Dial, M. E. Field, Robert J Collier, M. L. Rhoads

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The aim of this experiment was to localize the mRNA and protein of ghrelin and its active receptor, growth hormone secretagogue 1A (GHS-R1A), within the reproductive tract of dairy cattle. Ghrelin is an orexigenic hormone that has been identified as a potent regulator of energy homeostasis. Recent evidence suggests that ghrelin may also serve as a metabolic signal to the reproductive tract. Ghrelin and GHS-R1A have been identified in the reproductive tract of several species, including humans, mice, and rats. However, ghrelin and GHS-R1A expression have not been described within bovine reproductive tissues. Therefore, the ampulla, isthmus, uterine body, corpus luteum, and follicles were harvested from 3 Holstein heifers (15.91±0.07. mo of age) immediately following exsanguination. Duodenum and hypothalamus were collected as positive controls for ghrelin and GHS-R1A, respectively. Tissues were fixed in 10% formalin and embedded in paraffin for microscopy. Additional samples were stored at -80°C for detection of mRNA. Ghrelin and GHS-R1A mRNA and protein were observed in all tissue types within the reproductive tract of dairy heifers; however, expression appeared to be cell specific. Furthermore, ghrelin protein appeared to be localized to the cytoplasm, whereas GHS-R1A protein was found on the plasma membrane. Within the reproductive tissues, ghrelin mRNA and protein were most abundantly expressed in the ampulla of the oviduct. Concentrations of GHS-R1A were lower than those of ghrelin but differed between tissues. This is one of the first studies to provide molecular evidence for the presence of ghrelin and GHS-R1A within the entire reproductive tract. However, implications for fertility remain to be determined.

Original languageEnglish (US)
Pages (from-to)150-157
Number of pages8
JournalJournal of Dairy Science
Volume96
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint

Ghrelin Receptor
ghrelin
Ghrelin
heifers
Holstein
receptors
somatotropin
Growth Hormone
Messenger RNA
Proteins
proteins
Exsanguination
dairy heifers
Oviducts
Corpus Luteum
hypothalamus
oviducts
corpus luteum
duodenum
Duodenum

Keywords

  • Dairy cattle
  • Ghrelin
  • Ghrelin receptor (GHS-R1A)

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • Genetics

Cite this

Localization of ghrelin and its receptor in the reproductive tract of Holstein heifers. / Deaver, S. E.; Hoyer, Patricia B; Dial, S. M.; Field, M. E.; Collier, Robert J; Rhoads, M. L.

In: Journal of Dairy Science, Vol. 96, No. 1, 01.2013, p. 150-157.

Research output: Contribution to journalArticle

Deaver, S. E. ; Hoyer, Patricia B ; Dial, S. M. ; Field, M. E. ; Collier, Robert J ; Rhoads, M. L. / Localization of ghrelin and its receptor in the reproductive tract of Holstein heifers. In: Journal of Dairy Science. 2013 ; Vol. 96, No. 1. pp. 150-157.
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