Localization of myoinhibitory peptide immunoreactivity in Manduca sexta and Bombyx mori, with indications that the peptide has a role in molting and ecdysis

Norman T. Davis, Michael B. Blackburn, Elena G. Golubeva, John G Hildebrand

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

For normal development of Manduca sexta larvae, the ecdysteroid titer must drop following its sudden rise at the start of the molting cycle; this sudden decline in titer may be due to myoinhibitory peptide I (MIP I), which has an inhibitory effect on the release of ecdysone by the prothoracic glands of Bombyx mori in vitro. Using an antiserum to MIP, we have demonstrated secretion of an MIP-like peptide by the epiproctodeal glands of Manduca sexta, which are located on each proctodeal nerve, just anterior to the rectum. These MIP-immunoreactive glands are also present in B. mori. In fourth-instar larvae of M. sexta, the epiproctodeal glands show a distinct cycle of synthesis and sudden release of MIP that coincides with the time of the rapid decline in ecdysteroid titer. The function of the epiproctodeal glands appears to be the timely release of MIP during the molting cycle, so as to inhibit the prothoracic glands and thus to facilitate the sudden decline in ecdysteroid titer. In addition, we have found that MIP immunoreactivity is co-localized with that of crustacean cardioactive peptide (CCAP) in the 704 interneurons; these peptides appear to be co-released at the time of ecdysis. It is known that CCAP can initiate the ecdysis motor program; our results suggest that MIP may also be involved in activating ecdysis behavior.

Original languageEnglish (US)
Pages (from-to)1449-1460
Number of pages12
JournalJournal of Experimental Biology
Volume206
Issue number9
DOIs
StatePublished - May 2003

Fingerprint

Manduca
Molting
Bombyx
ecdysis
Manduca sexta
Bombyx mori
peptide
molting
Ecdysteroids
peptides
ecdysteroids
Peptides
prothoracic glands
Larva
crustacean
Crustacea
larva
Ecdysone
ecdysone
larvae

Keywords

  • Allatostatin
  • Bombyx mori
  • CCAP
  • Ecdysteroids
  • Ecdysterostatic hormone
  • Epiproctodeal gland
  • Manduca sexta
  • Neurosecretion
  • Prothoracicostatic peptide
  • Tobacco hornworm

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Localization of myoinhibitory peptide immunoreactivity in Manduca sexta and Bombyx mori, with indications that the peptide has a role in molting and ecdysis. / Davis, Norman T.; Blackburn, Michael B.; Golubeva, Elena G.; Hildebrand, John G.

In: Journal of Experimental Biology, Vol. 206, No. 9, 05.2003, p. 1449-1460.

Research output: Contribution to journalArticle

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abstract = "For normal development of Manduca sexta larvae, the ecdysteroid titer must drop following its sudden rise at the start of the molting cycle; this sudden decline in titer may be due to myoinhibitory peptide I (MIP I), which has an inhibitory effect on the release of ecdysone by the prothoracic glands of Bombyx mori in vitro. Using an antiserum to MIP, we have demonstrated secretion of an MIP-like peptide by the epiproctodeal glands of Manduca sexta, which are located on each proctodeal nerve, just anterior to the rectum. These MIP-immunoreactive glands are also present in B. mori. In fourth-instar larvae of M. sexta, the epiproctodeal glands show a distinct cycle of synthesis and sudden release of MIP that coincides with the time of the rapid decline in ecdysteroid titer. The function of the epiproctodeal glands appears to be the timely release of MIP during the molting cycle, so as to inhibit the prothoracic glands and thus to facilitate the sudden decline in ecdysteroid titer. In addition, we have found that MIP immunoreactivity is co-localized with that of crustacean cardioactive peptide (CCAP) in the 704 interneurons; these peptides appear to be co-released at the time of ecdysis. It is known that CCAP can initiate the ecdysis motor program; our results suggest that MIP may also be involved in activating ecdysis behavior.",
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