Localization of neurons expressing substance P and neurokinin B gene transcripts in the human hypothalamus and basal forebrain

Monica K. Chawla, Graciela M. Gutierrez, W. Scott Young, Nathaniel T. Mcmullen, Naomi E Rance

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

In situ hybridization histochemistry was used to map the distribution of neurons expressing the substance P (SP) or neurokinin B (NKB) genes in the human hypothalamus and basal forebrain. Hypothalami from five adult males were frozen in isopentane at -30°C and serially sectioned at 20 μm thickness. Every 20th section was hybridized with [35S]labeled, 48-base synthetic cDNA probes that were complementary to either SP or NKB mRNAs. Slides were dipped into nuclear emulsion for visualization of mRNAs at the single-cell level. The location of labeled neurons (greater than X 5 background) was mapped by using an image-combining computer microscope system. A distinct and complementary distribution pattern of SP and NKB neurons was observed in the human hypothalamus and basal forebrain. NKB was the predominant tachykinin in the rostral hypothalamus, whereas SP mRNA predominated in the posterior hypothalamus. Numerous NKB neurons were identified in the magnocellular basal forebrain, the bed nucleus of stria terminalis, and the anterior hypothalamic area. Scattered NKB neurons were present in the infundibular and paraventricular nuclei, paraolfactory gyrus, posterior hypothalamic area, lateral division of the medial mammillary nucleus, and amygdala. Numerous neurons expressing SP mRNAs were identified in the premammillary, supramammillary, and medial mammillary nuclei; the posterior hypothalamic area; and the corpus striatum. Scattered SP neurons were also observed in the preoptic area; the infundibular, intermediate, dorsomedial, and ventromedial nuclei; the infundibular stalk; the amygdala; the bed nucleus of stria terminalis; and the paraolfactory gyrus. These studies provide the first description of the location of neurons that express tachykinin gene transcripts in the human hypothalamus.

Original languageEnglish (US)
Pages (from-to)429-442
Number of pages14
JournalJournal of Comparative Neurology
Volume384
Issue number3
DOIs
StatePublished - Aug 4 1997

Fingerprint

Neurokinin B
Substance P
Hypothalamus
Neurons
Genes
Tachykinins
Septal Nuclei
Messenger RNA
Anterior Hypothalamic Nucleus
Lateral Hypothalamic Area
Posterior Hypothalamus
Mediodorsal Thalamic Nucleus
Corpus Striatum
Arcuate Nucleus of Hypothalamus
Preoptic Area
Basal Forebrain
Paraventricular Hypothalamic Nucleus
Computer Systems
Pituitary Gland
Amygdala

Keywords

  • Diagonal band of Broca
  • In situ hybridization
  • Mammillary body
  • Nucleus basalis of Meynert
  • Putamen
  • Tachykinin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Localization of neurons expressing substance P and neurokinin B gene transcripts in the human hypothalamus and basal forebrain. / Chawla, Monica K.; Gutierrez, Graciela M.; Young, W. Scott; Mcmullen, Nathaniel T.; Rance, Naomi E.

In: Journal of Comparative Neurology, Vol. 384, No. 3, 04.08.1997, p. 429-442.

Research output: Contribution to journalArticle

Chawla, Monica K. ; Gutierrez, Graciela M. ; Young, W. Scott ; Mcmullen, Nathaniel T. ; Rance, Naomi E. / Localization of neurons expressing substance P and neurokinin B gene transcripts in the human hypothalamus and basal forebrain. In: Journal of Comparative Neurology. 1997 ; Vol. 384, No. 3. pp. 429-442.
@article{ad66bd0ce499430ca7129020192d9350,
title = "Localization of neurons expressing substance P and neurokinin B gene transcripts in the human hypothalamus and basal forebrain",
abstract = "In situ hybridization histochemistry was used to map the distribution of neurons expressing the substance P (SP) or neurokinin B (NKB) genes in the human hypothalamus and basal forebrain. Hypothalami from five adult males were frozen in isopentane at -30°C and serially sectioned at 20 μm thickness. Every 20th section was hybridized with [35S]labeled, 48-base synthetic cDNA probes that were complementary to either SP or NKB mRNAs. Slides were dipped into nuclear emulsion for visualization of mRNAs at the single-cell level. The location of labeled neurons (greater than X 5 background) was mapped by using an image-combining computer microscope system. A distinct and complementary distribution pattern of SP and NKB neurons was observed in the human hypothalamus and basal forebrain. NKB was the predominant tachykinin in the rostral hypothalamus, whereas SP mRNA predominated in the posterior hypothalamus. Numerous NKB neurons were identified in the magnocellular basal forebrain, the bed nucleus of stria terminalis, and the anterior hypothalamic area. Scattered NKB neurons were present in the infundibular and paraventricular nuclei, paraolfactory gyrus, posterior hypothalamic area, lateral division of the medial mammillary nucleus, and amygdala. Numerous neurons expressing SP mRNAs were identified in the premammillary, supramammillary, and medial mammillary nuclei; the posterior hypothalamic area; and the corpus striatum. Scattered SP neurons were also observed in the preoptic area; the infundibular, intermediate, dorsomedial, and ventromedial nuclei; the infundibular stalk; the amygdala; the bed nucleus of stria terminalis; and the paraolfactory gyrus. These studies provide the first description of the location of neurons that express tachykinin gene transcripts in the human hypothalamus.",
keywords = "Diagonal band of Broca, In situ hybridization, Mammillary body, Nucleus basalis of Meynert, Putamen, Tachykinin",
author = "Chawla, {Monica K.} and Gutierrez, {Graciela M.} and Young, {W. Scott} and Mcmullen, {Nathaniel T.} and Rance, {Naomi E}",
year = "1997",
month = "8",
day = "4",
doi = "10.1002/(SICI)1096-9861(19970804)384:3<429::AID-CNE8>3.0.CO;2-5",
language = "English (US)",
volume = "384",
pages = "429--442",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Localization of neurons expressing substance P and neurokinin B gene transcripts in the human hypothalamus and basal forebrain

AU - Chawla, Monica K.

AU - Gutierrez, Graciela M.

AU - Young, W. Scott

AU - Mcmullen, Nathaniel T.

AU - Rance, Naomi E

PY - 1997/8/4

Y1 - 1997/8/4

N2 - In situ hybridization histochemistry was used to map the distribution of neurons expressing the substance P (SP) or neurokinin B (NKB) genes in the human hypothalamus and basal forebrain. Hypothalami from five adult males were frozen in isopentane at -30°C and serially sectioned at 20 μm thickness. Every 20th section was hybridized with [35S]labeled, 48-base synthetic cDNA probes that were complementary to either SP or NKB mRNAs. Slides were dipped into nuclear emulsion for visualization of mRNAs at the single-cell level. The location of labeled neurons (greater than X 5 background) was mapped by using an image-combining computer microscope system. A distinct and complementary distribution pattern of SP and NKB neurons was observed in the human hypothalamus and basal forebrain. NKB was the predominant tachykinin in the rostral hypothalamus, whereas SP mRNA predominated in the posterior hypothalamus. Numerous NKB neurons were identified in the magnocellular basal forebrain, the bed nucleus of stria terminalis, and the anterior hypothalamic area. Scattered NKB neurons were present in the infundibular and paraventricular nuclei, paraolfactory gyrus, posterior hypothalamic area, lateral division of the medial mammillary nucleus, and amygdala. Numerous neurons expressing SP mRNAs were identified in the premammillary, supramammillary, and medial mammillary nuclei; the posterior hypothalamic area; and the corpus striatum. Scattered SP neurons were also observed in the preoptic area; the infundibular, intermediate, dorsomedial, and ventromedial nuclei; the infundibular stalk; the amygdala; the bed nucleus of stria terminalis; and the paraolfactory gyrus. These studies provide the first description of the location of neurons that express tachykinin gene transcripts in the human hypothalamus.

AB - In situ hybridization histochemistry was used to map the distribution of neurons expressing the substance P (SP) or neurokinin B (NKB) genes in the human hypothalamus and basal forebrain. Hypothalami from five adult males were frozen in isopentane at -30°C and serially sectioned at 20 μm thickness. Every 20th section was hybridized with [35S]labeled, 48-base synthetic cDNA probes that were complementary to either SP or NKB mRNAs. Slides were dipped into nuclear emulsion for visualization of mRNAs at the single-cell level. The location of labeled neurons (greater than X 5 background) was mapped by using an image-combining computer microscope system. A distinct and complementary distribution pattern of SP and NKB neurons was observed in the human hypothalamus and basal forebrain. NKB was the predominant tachykinin in the rostral hypothalamus, whereas SP mRNA predominated in the posterior hypothalamus. Numerous NKB neurons were identified in the magnocellular basal forebrain, the bed nucleus of stria terminalis, and the anterior hypothalamic area. Scattered NKB neurons were present in the infundibular and paraventricular nuclei, paraolfactory gyrus, posterior hypothalamic area, lateral division of the medial mammillary nucleus, and amygdala. Numerous neurons expressing SP mRNAs were identified in the premammillary, supramammillary, and medial mammillary nuclei; the posterior hypothalamic area; and the corpus striatum. Scattered SP neurons were also observed in the preoptic area; the infundibular, intermediate, dorsomedial, and ventromedial nuclei; the infundibular stalk; the amygdala; the bed nucleus of stria terminalis; and the paraolfactory gyrus. These studies provide the first description of the location of neurons that express tachykinin gene transcripts in the human hypothalamus.

KW - Diagonal band of Broca

KW - In situ hybridization

KW - Mammillary body

KW - Nucleus basalis of Meynert

KW - Putamen

KW - Tachykinin

UR - http://www.scopus.com/inward/record.url?scp=0030815958&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030815958&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9861(19970804)384:3<429::AID-CNE8>3.0.CO;2-5

DO - 10.1002/(SICI)1096-9861(19970804)384:3<429::AID-CNE8>3.0.CO;2-5

M3 - Article

C2 - 9254037

AN - SCOPUS:0030815958

VL - 384

SP - 429

EP - 442

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 3

ER -