Localized perturbations in CheY structure monitored by NMR identify a CheA binding interface

R. V. Swanson, D. F. Lowry, P. Matsumura, Megan McEvoy, M. I. Simon, F. W. Dahlquist

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Phosphotransfer between the autophosphorylating histidine kinase CheA and the response regulator CheY represents a crucial step in the bacterial chemotaxis signal transduction pathway. The 15N-1H correlation spectrum of CheY complexed with an amino-terminal fragment of CheA exhibits specific localized differences in chemical shifts when compared to the spectrum of uncomplexed CheY. When mapped onto the three-dimensional structure of CheY, these changes define a region distinct from the active site. A single amino- acid substitution within this binding region on CheY, alanine to valine at position 103, significantly decreases the affinity of CheY for CheA. The binding face described by these changes partially overlaps a flagellar switch binding surface previously defined by mutagenesis.

Original languageEnglish (US)
Pages (from-to)906-910
Number of pages5
JournalNature Structural Biology
Volume2
Issue number10
StatePublished - 1995
Externally publishedYes

Fingerprint

Signal transduction
Mutagenesis
Valine
Chemical shift
Chemotaxis
Amino Acid Substitution
Histidine
Alanine
Signal Transduction
Catalytic Domain
Substitution reactions
Phosphotransferases
Switches
Nuclear magnetic resonance
Amino Acids
Histidine Kinase

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology

Cite this

Swanson, R. V., Lowry, D. F., Matsumura, P., McEvoy, M., Simon, M. I., & Dahlquist, F. W. (1995). Localized perturbations in CheY structure monitored by NMR identify a CheA binding interface. Nature Structural Biology, 2(10), 906-910.

Localized perturbations in CheY structure monitored by NMR identify a CheA binding interface. / Swanson, R. V.; Lowry, D. F.; Matsumura, P.; McEvoy, Megan; Simon, M. I.; Dahlquist, F. W.

In: Nature Structural Biology, Vol. 2, No. 10, 1995, p. 906-910.

Research output: Contribution to journalArticle

Swanson, RV, Lowry, DF, Matsumura, P, McEvoy, M, Simon, MI & Dahlquist, FW 1995, 'Localized perturbations in CheY structure monitored by NMR identify a CheA binding interface', Nature Structural Biology, vol. 2, no. 10, pp. 906-910.
Swanson, R. V. ; Lowry, D. F. ; Matsumura, P. ; McEvoy, Megan ; Simon, M. I. ; Dahlquist, F. W. / Localized perturbations in CheY structure monitored by NMR identify a CheA binding interface. In: Nature Structural Biology. 1995 ; Vol. 2, No. 10. pp. 906-910.
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