Location-dependent coronary artery diffusive and convective mass transport properties of a lipophilic drug surrogate measured using nonlinear microscopy

Joseph T. Keyes, Bruce R. Simon, Jonathan P Vande Geest

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: Arterial wall mass transport properties dictate local distribution of biomolecules or locally delivered dugs. Knowing how these properties vary between coronary artery locations could provide insight into how therapy efficacy is altered between arterial locations. Methods: We introduced an indocarbocyanine drug surrogate to the lumens of left anterior descending and right coronary (LADC; RC) arteries from pigs with or without a pressure gradient. Interstitial fluorescent intensity was measured on live samples with multiphoton microscopy. We also measured binding to porcine coronary SMCs in monoculture. Results: Diffusive transport constants peaked in the middle sections of the LADC and RC arteries by 2.09 and 2.04 times, respectively, compared to the proximal and distal segments. There was no statistical difference between the average diffusivity value between LADC and RC arteries. The convection coefficients had an upward trend down each artery, with the RC being higher than the LADC by 3.89 times. Conclusions: This study demonstrates that the convective and diffusive transport of lipophilic molecules changes between the LADC and the RC arteries as well as along their length. These results may have important implications in optimizing drug delivery for the treatment of coronary artery disease.

Original languageEnglish (US)
Pages (from-to)1147-1160
Number of pages14
JournalPharmaceutical Research
Volume30
Issue number4
DOIs
StatePublished - Apr 2013

Fingerprint

Transport properties
Microscopy
Coronary Vessels
Microscopic examination
Mass transfer
Arteries
Biomolecules
Pressure gradient
Drug delivery
Pharmaceutical Preparations
Swine
Convection
Molecules
Coronary Artery Disease
Pressure
Therapeutics

Keywords

  • convection
  • coronary
  • DiI
  • porcine
  • transport

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Organic Chemistry
  • Molecular Medicine
  • Pharmacology (medical)
  • Biotechnology
  • Pharmacology

Cite this

Location-dependent coronary artery diffusive and convective mass transport properties of a lipophilic drug surrogate measured using nonlinear microscopy. / Keyes, Joseph T.; Simon, Bruce R.; Vande Geest, Jonathan P.

In: Pharmaceutical Research, Vol. 30, No. 4, 04.2013, p. 1147-1160.

Research output: Contribution to journalArticle

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