Locomotive forces produced by single leukocytes in vivo and in vitro

W. H. Guilford, Robert Clark Lantz, R. W. Gore

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We report here the first time-resolved measurements of the forces produced during the migration of single leukocytes in vivo and in vitro. Pulmonary macrophages from hamsters and mice, in vitro, and Nembutal (pentobarbital sodium)-anesthetized hamster neutrophils, in vivo, generated maximum locomotive forces ranging from 1.9 to 10.7 nN or tenths of microdynes. Force production was periodic and correlated with the length of the leading lamellipod but not with generalized cell ruffling. Although the extension of the leading lamella is critical to locomotive force generation, these direct measurements suggest that lamellar extension may not arise from the same contractile processes driving forward motion of the cell mass. Indeed, cell ruffling, lamellar extension, and locomotive force generation may be differentially controlled and have different origins. This technique may be extended to test numerous hypotheses of how these and other nonmuscle cells crawl.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume268
Issue number5 37-5
StatePublished - 1995

Fingerprint

Locomotives
leukocytes
Leukocytes
Pentobarbital
Cricetinae
hamsters
Time measurement
cells
Alveolar Macrophages
pentobarbital
Neutrophils
neutrophils
macrophages
lungs
In Vitro Techniques
sodium
mice
testing

Keywords

  • actin
  • leading lamella
  • macrophage
  • motility
  • myosin
  • neutrophil

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Locomotive forces produced by single leukocytes in vivo and in vitro. / Guilford, W. H.; Lantz, Robert Clark; Gore, R. W.

In: American Journal of Physiology - Cell Physiology, Vol. 268, No. 5 37-5, 1995.

Research output: Contribution to journalArticle

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