Lopinavir protein binding in HIV-1-infected pregnant women

F. T. Aweeka, A. Stek, B. M. Best, C. Hu, D. Holland, A. Hermes, S. K. Burchett, J. Read, M. Mirochnick, E. V. Capparelli

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Background: Pregnancy may alter protein binding (PB) of highly bound protease inhibitors due to changes in plasma concentrations of albumin and α-1 acid glycoprotein (AAG). Small changes in PB can greatly impact the fraction of drug unbound (FU) exerting pharmacological effect. We report lopinavir (LPV) PB during third trimester (antepartum, AP) compared to ≥1.7 weeks postpartum (PP) to determine if FU changes compensate for reduced total concentrations reported previously. Methods: P1026s enrolled women receiving LPV/ritonavir, soft gel capsules 400/100 mg or 533/133 mg twice daily. LPV FU, albumin and AAG were determined AP and PP. Results:AP/PP samples were available from 29/25 women respectively with all but one woman receiving the same dose AP/PP. LPV FU was increased 18% AP vs. PP (mean 0.96±0.16% AP vs. 0.82±0.21% PP, P=0.001). Mean protein concentrations were reduced AP (AAG=477 mg/L; albumin=3.28 mg/dL) vs. PP (AAG=1007 mg/L; albumin=3.85 mg/dL) (P<0.0001 for each comparison). AAG concentration correlated with LPV binding. Total LPV concentration did not correlate with LPV FU AP or PP. However, higher LPV concentration PP was associated with reduced PB and higher FU after adjustment for AAG. Conclusions:LPV FU was higher and AAG lower AP vs. PP. The 18% increase in LPV FU AP is smaller than the reduction in total LPV concentration reported previously and is not of sufficient magnitude to eliminate the need for an increased dose during pregnancy.

Original languageEnglish (US)
Pages (from-to)232-238
Number of pages7
JournalHIV Medicine
Volume11
Issue number4
DOIs
StatePublished - Apr 2010

Keywords

  • Lopinavir
  • Pharmacokinetics
  • Plasma proteins
  • Pregnancy
  • Protein binding

ASJC Scopus subject areas

  • Health Policy
  • Infectious Diseases
  • Pharmacology (medical)

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  • Cite this

    Aweeka, F. T., Stek, A., Best, B. M., Hu, C., Holland, D., Hermes, A., Burchett, S. K., Read, J., Mirochnick, M., & Capparelli, E. V. (2010). Lopinavir protein binding in HIV-1-infected pregnant women. HIV Medicine, 11(4), 232-238. https://doi.org/10.1111/j.1468-1293.2009.00767.x