Loss of HSF1 results in defective radiation-induced G2 arrest and DNA repair

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

HSF1 is a transcription factor that plays a key role in the response to heat stress and was previously shown by us to also be essential for importation of p53 into the nucleus. Here we extend these studies and show that loss of HSF1 renders cells more sensitive to killing by ionizing radiation. Cells that lack a functional HSF1 were unable to arrest in G2 after exposure to ionizing radiation, suggesting that HSF1 activity was essential for activation of this cell cycle checkpoint. In addition, cells with no HSF1 showed a reduced capacity to repair radiation-induced double-stranded DNA breaks. We found that in these cells 53BP1 did not accumulate at sites of DNA damage, suggesting that HSF1 was also essential for the functioning of this DNA damage mediator. Taken together our results indicate that HSF1 plays an important role in checkpoint activation and DNA repair and suggest that there is overlap between the heat stress response pathway and the pathway that responds to ionizing radiation.

Original languageEnglish (US)
Pages (from-to)17-24
Number of pages8
JournalRadiation Research
Volume176
Issue number1
DOIs
StatePublished - Jul 2011

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DNA Repair
deoxyribonucleic acid
Ionizing Radiation
ionizing radiation
Radiation
Heat-Shock Response
radiation
cells
DNA Damage
activation
damage
heat
Double-Stranded DNA Breaks
Cell Cycle Checkpoints
Transcription Factors
cycles
nuclei

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Loss of HSF1 results in defective radiation-induced G2 arrest and DNA repair. / Li, Qiang; Martinez, Jesse D.

In: Radiation Research, Vol. 176, No. 1, 07.2011, p. 17-24.

Research output: Contribution to journalArticle

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