Nizkoaffinnyǐ retseptor IgE cheloveka

klonirovanie kDNK iz B-limfoblastoidnoǐ kletochnoǐ linii 1B i izychenie ego ékspressii v B-kletkakh perifericheskoǐ krovi.

Translated title of the contribution: Low affinity human IgE receptor: cloning cDNA from B-lymphocytes of cell line 1B and study of its expression in peripheral blood B-cells

Vadim I Pivniouk, L. K. Chelidze, E. O. Martsen, E. N. Tsytsikov, R. G. Vasilov

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Previously, the cDNA coding for human low-affinity receptor for IgE (FcERII/CD23) was cloned by three independent groups. Using oligonucleotide probes corresponding to the published cDNA sequence of CD23, we have isolated cDNA clones encoding FcERII from the cDNA library of B-lymphoblastoid cell line 1B obtained in our laboratory. Cloned nucleotide sequences of FcERII were shown to be identical to those from the RPMI8866 cell line. Using a DNA fragment containing the full-length cDNA copy of CD23 as a probe, CD23 expression in highly purified peripheral blood B lymphocytes was shown to be induced by recombinant human IL4 in a clearly dose-dependent manner.

Original languageRussian
Pages (from-to)840-845
Number of pages6
JournalMolekulyarnaya Biologiya
Volume28
Issue number4
StatePublished - Jul 1994
Externally publishedYes

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IgE Receptors
Organism Cloning
Blood Cells
B-Lymphocytes
Complementary DNA
Cell Line
Oligonucleotide Probes
Gene Library
Interleukin-4
Clone Cells
DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Nizkoaffinnyǐ retseptor IgE cheloveka : klonirovanie kDNK iz B-limfoblastoidnoǐ kletochnoǐ linii 1B i izychenie ego ékspressii v B-kletkakh perifericheskoǐ krovi. / Pivniouk, Vadim I; Chelidze, L. K.; Martsen, E. O.; Tsytsikov, E. N.; Vasilov, R. G.

In: Molekulyarnaya Biologiya, Vol. 28, No. 4, 07.1994, p. 840-845.

Research output: Contribution to journalArticle

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abstract = "Previously, the cDNA coding for human low-affinity receptor for IgE (FcERII/CD23) was cloned by three independent groups. Using oligonucleotide probes corresponding to the published cDNA sequence of CD23, we have isolated cDNA clones encoding FcERII from the cDNA library of B-lymphoblastoid cell line 1B obtained in our laboratory. Cloned nucleotide sequences of FcERII were shown to be identical to those from the RPMI8866 cell line. Using a DNA fragment containing the full-length cDNA copy of CD23 as a probe, CD23 expression in highly purified peripheral blood B lymphocytes was shown to be induced by recombinant human IL4 in a clearly dose-dependent manner.",
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AU - Chelidze, L. K.

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AU - Tsytsikov, E. N.

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