Low-cost, high-speed near infrared reflectance confocal microscope

Cheng Gong, Nachiket Kulkarni, Wenbin Zhu, Christopher David Nguyen, Clara N Curiel, Dongkyun Kang

Research output: Contribution to journalArticle

Abstract

We have developed a low-cost, near-infrared (NIR) reflectance confocal microscope (RCM) to overcome challenges in the imaging depth and speed found in our previously-reported smartphone confocal microscope. In the new NIR RCM device, we have used 840 nm superluminescent LED (sLED) to increase the tissue imaging depth and speed. A new confocal detection optics has been developed to maintain high lateral resolution even when a relatively large slit width was used. The material cost of the NIR RCM device was still low, ~$5,200. The lateral resolution was 1.1 µm and 1.3 µm along the vertical and horizontal directions, respectively. Axial resolution was measured as 11.2 µm. In vivo confocal images of human forearm skin obtained at the imaging speed of 203 frames/sec clearly visualized characteristic epidermal and dermal cellular features of the human skin.

Original languageEnglish (US)
Article number#363896
Pages (from-to)3497-3505
Number of pages9
JournalBiomedical Optics Express
Volume10
Issue number7
DOIs
StatePublished - Jul 1 2019

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microscopes
high speed
reflectance
Costs and Cost Analysis
Skin
Equipment and Supplies
forearm
Forearm
slits
light emitting diodes
optics
costs
Direction compound
Smartphone

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Gong, C., Kulkarni, N., Zhu, W., Nguyen, C. D., Curiel, C. N., & Kang, D. (2019). Low-cost, high-speed near infrared reflectance confocal microscope. Biomedical Optics Express, 10(7), 3497-3505. [#363896]. https://doi.org/10.1364/BOE.10.003497

Low-cost, high-speed near infrared reflectance confocal microscope. / Gong, Cheng; Kulkarni, Nachiket; Zhu, Wenbin; Nguyen, Christopher David; Curiel, Clara N; Kang, Dongkyun.

In: Biomedical Optics Express, Vol. 10, No. 7, #363896, 01.07.2019, p. 3497-3505.

Research output: Contribution to journalArticle

Gong, C, Kulkarni, N, Zhu, W, Nguyen, CD, Curiel, CN & Kang, D 2019, 'Low-cost, high-speed near infrared reflectance confocal microscope', Biomedical Optics Express, vol. 10, no. 7, #363896, pp. 3497-3505. https://doi.org/10.1364/BOE.10.003497
Gong, Cheng ; Kulkarni, Nachiket ; Zhu, Wenbin ; Nguyen, Christopher David ; Curiel, Clara N ; Kang, Dongkyun. / Low-cost, high-speed near infrared reflectance confocal microscope. In: Biomedical Optics Express. 2019 ; Vol. 10, No. 7. pp. 3497-3505.
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