Low-dose immunosuppression in a rat hind-limb transplantation model

Gustavo Perez-Abadia, Luis Laurentin-Perez, Vijay S. Gorantla, Cedric G. Francois, Marieke Vossen, Pascal C.R. Brouha, Haldun I. Orhun, Gary L. Anderson, Claudio Maldonado, Diane J. Pidwell, Warren C. Breidenbach, John H. Barker

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Composite tissue allografts (CTAs) offer an alternative to conventional reconstructive methods. However, the toxicity of the drugs that are required to prevent rejection has prevented its widespread clinical application. The purpose of this study was to determine whether a low-dose, corticosteroid-free combination regimen of tacrolimus and mycophenolate mofetil (MMF) would prevent rejection in a rat hind-limb model, with minimal toxic side effects. Three groups were used in this study. In group I, Wistar Furth (WF) rats received a syngeneic WF hind-limb. In groups II and III, WF rats received an ACI hind-limb. The latter were treated with tacrolimus-MMF. Assessment for rejection, flow cytometry, and mixed lymphocyte reactions was performed. Biopsies were taken regularly and at the time of killing. Combination therapy with low-dose tacrolimus-MMF effectively prolonged CTA survival indefinitely, with minimal side effects. Toxicity associated with immunosuppressive drugs can be avoided in a low-dose combination corticosteroid-free regimen.

Original languageEnglish (US)
Pages (from-to)835-842
Number of pages8
JournalTransplant International
Volume16
Issue number12
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

Keywords

  • Combination therapy
  • Composite tissue allografts
  • Mycophenolate mofetil
  • Rats
  • Tacrolimus

ASJC Scopus subject areas

  • Transplantation

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  • Cite this

    Perez-Abadia, G., Laurentin-Perez, L., Gorantla, V. S., Francois, C. G., Vossen, M., Brouha, P. C. R., Orhun, H. I., Anderson, G. L., Maldonado, C., Pidwell, D. J., Breidenbach, W. C., & Barker, J. H. (2003). Low-dose immunosuppression in a rat hind-limb transplantation model. Transplant International, 16(12), 835-842. https://doi.org/10.1007/s00147-003-0576-4