Low-frequency mode activity of heme: Femtosecond coherence spectroscopy of iron porphine halides and nitrophorin

Minoru Kubo, Flaviu Gruia, Abdelkrim Benabbas, Alexander Barabanschikov, William "Bill" Montfort, Estelle M. Maes, Paul M. Champion

Research output: Contribution to journalArticle

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Abstract

The low-frequency mode activity of metalloporphyrins has been studied for iron porphine-halides (Fe(P)(X), X = Cl, Br) and nitrophorin 4 (NP4) using femtosecond coherence spectroscopy (FCS) in combination with polarized resonance Raman spectroscopy and density functional theory (DFT). It is confirmed that the mode symmetry selection rules for FCS are the same as for Raman scattering and that both Franck-Condon and Jahn-Teller mode activities are observed for Fe(P)(X) under Soret resonance conditions. The DFT-calculated low-frequency (20-400 cm-1) modes, and their frequency shifts upon halide substitution, are in good agreement with experimental Raman and coherence data, so that mode assignments can be made. The doming mode is located at ∼80 cm-1 for Fe(P)(Cl) and at ∼60 cm-1 for Fe(P)(Br). NP4 is also studied with coherence techniques, and the NO-bound species of ferric and ferrous NP4 display a mode at ∼30-40 cm-1 that is associated with transient heme doming motion following NO photolysis. The coherence spectra of three ferric derivatives of NP4 with different degrees of heme ruffling distortion are also investigated. We find a mode at ∼60 cm-1 whose relative intensity in the coherence spectra depends quadratically on the magnitude of the ruffling distortion. To quantitatively account for this correlation, a new "distortion-induced" Raman enhancement mechanism is presented. This mechanism is unique to low-frequency "soft modes" of the molecular framework that can be distorted by environmental forces. These results demonstrate the potential of FCS as a sensitive probe of dynamic and functionally important nonplanar heme vibrational excitations that are induced by the protein environmental forces or by the chemical reactions in the aqueous phase.

Original languageEnglish (US)
Pages (from-to)9800-9811
Number of pages12
JournalJournal of the American Chemical Society
Volume130
Issue number30
DOIs
StatePublished - Jul 30 2008

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Laser modes
Heme
Spectrum Analysis
Iron
Spectroscopy
Raman Spectrum Analysis
Metalloporphyrins
Photolysis
Density functional theory
nitrophorin
porphine
Raman spectroscopy
Raman scattering
Chemical reactions
Substitution reactions
Proteins
Derivatives

ASJC Scopus subject areas

  • Chemistry(all)

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Low-frequency mode activity of heme : Femtosecond coherence spectroscopy of iron porphine halides and nitrophorin. / Kubo, Minoru; Gruia, Flaviu; Benabbas, Abdelkrim; Barabanschikov, Alexander; Montfort, William "Bill"; Maes, Estelle M.; Champion, Paul M.

In: Journal of the American Chemical Society, Vol. 130, No. 30, 30.07.2008, p. 9800-9811.

Research output: Contribution to journalArticle

Kubo, Minoru ; Gruia, Flaviu ; Benabbas, Abdelkrim ; Barabanschikov, Alexander ; Montfort, William "Bill" ; Maes, Estelle M. ; Champion, Paul M. / Low-frequency mode activity of heme : Femtosecond coherence spectroscopy of iron porphine halides and nitrophorin. In: Journal of the American Chemical Society. 2008 ; Vol. 130, No. 30. pp. 9800-9811.
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AU - Kubo, Minoru

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AU - Barabanschikov, Alexander

AU - Montfort, William "Bill"

AU - Maes, Estelle M.

AU - Champion, Paul M.

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