Low-temperature storage of transplants at the light compensation point: air temperature and light intensity for growth suppression and quality preservation

Chieri Kubota, Toyoki Kozai

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25 Scopus citations


A method for storing transplants in vitro was developed using light compensation points in conjunction with low temperatures. Broccoli (Brassica oleracea L., cultivar 'Ryokurei') plantlets, aseptically germinated and cultured for 3 weeks in vitro, were used as model transplants. Culture conditions were: 23 °C air temperature, 160 μmol m-2 s-1 photosynthetic photon flux density (PPFD), and 3.6 air exchanges per hour of the vessel. Prior to storage, CO2 exchange rates of the plantlets were measured at 3, 5, 10, 15 and 25 °C air temperatures under 0 (darkness), 2, and 4 μmol m-2 s-1 PPFD to determine light compensation points of the plantlets cultured with or without 20 g l-1 sucrose in the medium. Plantlets were stored for 6 weeks at 5, 10, and 15 °C under either 0 or 2 μmol m-2 s-1 continuous PPFD, which was near their light compensation points at these temperatures. Results from the measurements of CO2 exchange rates showed that the light compensation points varied with air temperature and with medium sugar level. Plantlet dry weight during storage was best maintained by keeping CO2 exchange rate of the plantlets close to zero throughout the storage period. High transplant qualities were successfully preserved at light compensation points: 2 μmol m-2 s-1 PPFD at 5-10 °C without sugar, and at 5 °C with sugar in the medium. This method may also be applicable for storage of other crop transplants, plug seedlings and cuttings.

Original languageEnglish (US)
Pages (from-to)193-204
Number of pages12
JournalScientia Horticulturae
Issue number3-4
Publication statusPublished - 1995
Externally publishedYes



  • Brassica oleracea L.
  • Carbon balance
  • Environmental control
  • Growth regulation

ASJC Scopus subject areas

  • Horticulture
  • Plant Science

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