Lymphatic imaging in experimental filariasis using magnetic resonance

T. C. Case, Evan C Unger, Michael J Bernas, Marlys H Witte, C. L. Witte, G. McNeill, C. Crandall, R. Crandall

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

RATIONALE AND OBJECTIVES. To evaluate acquired lymphatic abnormalities caused by filariasis, the authors examined the peripheral lymphatic system in normal ferrets and those chronically infected with Brugia malayi using magnetic resonance imaging (MRI). The findings were compared with previously obtained lymphangioscintigraphic (LAS) images in ferrets both with and without experimental filariasis. METHODS. Fifteen ferrets (11 infected with B. malayi and four noninfected controls) underwent whole body coronal MRI using a quadrature transmission-receive head coil at 0.5 Tesla operating at a resonant frequency of 21.5 mHz for protons with a 25-cm field of view. RESULTS. In contrast to normal animals, infected ferrets showed dilated hindlimb dermal lymphatic collaterals, enlarged high-signal intensity groin lymph nodes with punctate low-signal intensity centers and separate low- signal intensity spots with irregular thin channels, suggestive of nests of viable adult nematodes within tortuous lymphatics and nodes. MRI correlated with the LAS findings, and the interpretations were supported by light, scanning electron, and video microscopy. CONCLUSIONS. T2-weighted MRI in conjunction with LAS accurately depicts the peripheral lymphatic system in filarial-infected ferrets. These two modalities are useful complementary techniques to examine disorders characterized by lymphatic insufficiency.

Original languageEnglish (US)
Pages (from-to)293-297
Number of pages5
JournalInvestigative Radiology
Volume27
Issue number4
StatePublished - 1992

Fingerprint

Filariasis
Ferrets
Magnetic Resonance Spectroscopy
Magnetic Resonance Imaging
Brugia malayi
Lymphatic System
Lymphatic Abnormalities
Video Microscopy
Groin
Hindlimb
Protons
Electron Microscopy
Lymph Nodes
Head
Light
Skin

Keywords

  • filariasis
  • lymphatic insufficiency
  • lymphscintigraphy
  • magnetic resonance

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Lymphatic imaging in experimental filariasis using magnetic resonance. / Case, T. C.; Unger, Evan C; Bernas, Michael J; Witte, Marlys H; Witte, C. L.; McNeill, G.; Crandall, C.; Crandall, R.

In: Investigative Radiology, Vol. 27, No. 4, 1992, p. 293-297.

Research output: Contribution to journalArticle

Case, TC, Unger, EC, Bernas, MJ, Witte, MH, Witte, CL, McNeill, G, Crandall, C & Crandall, R 1992, 'Lymphatic imaging in experimental filariasis using magnetic resonance', Investigative Radiology, vol. 27, no. 4, pp. 293-297.
Case, T. C. ; Unger, Evan C ; Bernas, Michael J ; Witte, Marlys H ; Witte, C. L. ; McNeill, G. ; Crandall, C. ; Crandall, R. / Lymphatic imaging in experimental filariasis using magnetic resonance. In: Investigative Radiology. 1992 ; Vol. 27, No. 4. pp. 293-297.
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AU - Case, T. C.

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AU - Bernas, Michael J

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AU - McNeill, G.

AU - Crandall, C.

AU - Crandall, R.

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N2 - RATIONALE AND OBJECTIVES. To evaluate acquired lymphatic abnormalities caused by filariasis, the authors examined the peripheral lymphatic system in normal ferrets and those chronically infected with Brugia malayi using magnetic resonance imaging (MRI). The findings were compared with previously obtained lymphangioscintigraphic (LAS) images in ferrets both with and without experimental filariasis. METHODS. Fifteen ferrets (11 infected with B. malayi and four noninfected controls) underwent whole body coronal MRI using a quadrature transmission-receive head coil at 0.5 Tesla operating at a resonant frequency of 21.5 mHz for protons with a 25-cm field of view. RESULTS. In contrast to normal animals, infected ferrets showed dilated hindlimb dermal lymphatic collaterals, enlarged high-signal intensity groin lymph nodes with punctate low-signal intensity centers and separate low- signal intensity spots with irregular thin channels, suggestive of nests of viable adult nematodes within tortuous lymphatics and nodes. MRI correlated with the LAS findings, and the interpretations were supported by light, scanning electron, and video microscopy. CONCLUSIONS. T2-weighted MRI in conjunction with LAS accurately depicts the peripheral lymphatic system in filarial-infected ferrets. These two modalities are useful complementary techniques to examine disorders characterized by lymphatic insufficiency.

AB - RATIONALE AND OBJECTIVES. To evaluate acquired lymphatic abnormalities caused by filariasis, the authors examined the peripheral lymphatic system in normal ferrets and those chronically infected with Brugia malayi using magnetic resonance imaging (MRI). The findings were compared with previously obtained lymphangioscintigraphic (LAS) images in ferrets both with and without experimental filariasis. METHODS. Fifteen ferrets (11 infected with B. malayi and four noninfected controls) underwent whole body coronal MRI using a quadrature transmission-receive head coil at 0.5 Tesla operating at a resonant frequency of 21.5 mHz for protons with a 25-cm field of view. RESULTS. In contrast to normal animals, infected ferrets showed dilated hindlimb dermal lymphatic collaterals, enlarged high-signal intensity groin lymph nodes with punctate low-signal intensity centers and separate low- signal intensity spots with irregular thin channels, suggestive of nests of viable adult nematodes within tortuous lymphatics and nodes. MRI correlated with the LAS findings, and the interpretations were supported by light, scanning electron, and video microscopy. CONCLUSIONS. T2-weighted MRI in conjunction with LAS accurately depicts the peripheral lymphatic system in filarial-infected ferrets. These two modalities are useful complementary techniques to examine disorders characterized by lymphatic insufficiency.

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