Lymphocyte Blastogenic Responses to Cultured Allogeneic Tumor Cells in Vitro1

Ralph J. Anderson, Charles M. Mcbride, Evan M. Hersh

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

For development and evaluation of systems through which tumor immunity might be evaluated in man, allogeneic tumor cell-lymphocyte interactions were studied with the one-way mixed culture technique. The critical importance of dose-response studies in the evaluation of this interaction was demonstrated. Maximal blastogenic lymphocyte responses were induced by 5 X 104irradiated tumor cells, and inhibition of blastogenesis was induced by 106tumor cells. The median maximal blastogenic responses produced by the tumor cells was 3, 700 cpm, compared to that produced by phytohemagglutinin (58, 000 cpm), streptolysin O (17, 400 cpm), streptokinase-streptodomase (13, 500 cpm), allogeneic lymphocytes (30, 500 cpm), or allogeneic irradiated lymphoblasts (51, 000 cpm). The inhibition of blastogenesis produced by higher doses of tumor cells was caused by a nondialyzable factor released into the supernatant of cultured, irradiated, or mitomycin-treated cells. This study indicates that inhibitors influence lymphocyte blastogenic responses to tumor cells in vitro and suggests that such factors may influence host response to tumors.

Original languageEnglish (US)
Pages (from-to)988-992
Number of pages5
JournalCancer Research
Volume32
Issue number5
StatePublished - May 1972

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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