Lymphoid reconstitution after autologous PBSC transplantation with FACS- sorted CD34+ hematopoietic progenitors

Catherine Bomberger, Meeta Singh-Jairam, Glenn Rodey, Anastasia Guerriero, Andrew M. Yeager, William H. Fleming, H. Kent Holland, Edmund K. Waller

Research output: Contribution to journalArticle

168 Scopus citations

Abstract

T-cell and B-cell reconstitution was studied in nine patients who received fluorescence activated cell sorter (FACS)-sorted autologous CD34+ hematopoietic progenitor cells (HPC). The mean numbers of T cells (CD3+), B cells (CD19+) and CD34+ HPC administered to each patient were .004, .002, and 1.8 x 106 cells/kg, respectively. After high-dose myeloablative chemotherapy (busulfan, cyclophosphamide, etoposide) CD34+ HPC were infused and lymphoid reconstitution was monitored using flow cytometry and reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of VDJ T-cell receptor (TcR) sequences. Restoration of normal numbers of peripheral blood T cells and B cells among recipients of FACS-sorted CD34+ HPC was delayed compared to recipients of non-T-cell-depleted PBSC autografts. In both patient groups, the circulating T cells were primarily CD4-, CD8+, αβ TcR+, and CD45RO+, CD45RA- during the first 2 months after transplant. Subsequent increases in the frequency of CD45RA+ CD45RO- T cells occurred at 2 to 3 months after transplant, suggesting maturation of CD34+ hematopoietic progenitors to 'naive' T cells. Analysis of the TcR repertoire after hematopoietic reconstitution demonstrated decreased diversity of Vβ TcR expression associated with global decreases in the absolute number of total peripheral blood T cells and most Vp TcR+ subsets. Three of nine recipients of FACS-sorted CD34+ HPC demonstrated significant increases in the percentage of γδ+ peripheral T cells and CD5+ B cells at 3 to 9 weeks after transplantation, and all patients had transient oligoclonal expansions of T cells expressing specific Vβ TcR. Transplantation with highly purified CD34+ HPC results in reduced diversity of the peripheral T-cell repertoire during the early post-transplant period compared with patients receiving unmanipulated or MoAb-depleted transplants.

Original languageEnglish (US)
Pages (from-to)2588-2600
Number of pages13
JournalBlood
Volume91
Issue number7
StatePublished - Apr 1 1998

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Bomberger, C., Singh-Jairam, M., Rodey, G., Guerriero, A., Yeager, A. M., Fleming, W. H., Holland, H. K., & Waller, E. K. (1998). Lymphoid reconstitution after autologous PBSC transplantation with FACS- sorted CD34+ hematopoietic progenitors. Blood, 91(7), 2588-2600.