Lysosomal proteolysis in distally or proximally denervated rat soleus muscle

Randi B. Weinstein, Michael J. Slentz, Kimberly Webster, Julie A. Takeuchi, Marc E Tischler

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We examined the mechanism of accelerated proteolysis in denervated rat soleus muscles. The soleus was denervated by severing either the tibial nerve (proximal, short stump) or sciatic nerve (distal, long stump) at 24, 48, 72, or 96 h before excision. Twenty-four hours after denervation, the extent of atrophy was similar for proximal and distal denervation, although lysosomal latency declined in both groups. After 48 and 72 h, denervation resulted in a decline in protein content, an increase in in vitro protein degradation, and a decline in lysosomal latency, all of which were greater in proximally denervated than in contralateral distally denervated muscles. These differences between acute responses of proximally and distally denervated muscles suggest the retention of some factor in the longer nerve stump that attenuates atrophy. After 96 h, total protein loss, protein degradation, and lysosomal latency were similar for proximal and distal denervation, suggesting the loss of axoplasmic flow from the long nerve stump.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume273
Issue number4 42-4
StatePublished - 1997

Fingerprint

Denervation
Proteolysis
Skeletal Muscle
Atrophy
Muscles
Tibial Nerve
Axonal Transport
Sciatic Nerve
Proteins

Keywords

  • Muscle atrophy
  • Neurotrophic factors
  • Sciatic nerve
  • Tibial nerve

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Lysosomal proteolysis in distally or proximally denervated rat soleus muscle. / Weinstein, Randi B.; Slentz, Michael J.; Webster, Kimberly; Takeuchi, Julie A.; Tischler, Marc E.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 273, No. 4 42-4, 1997.

Research output: Contribution to journalArticle

Weinstein, Randi B. ; Slentz, Michael J. ; Webster, Kimberly ; Takeuchi, Julie A. ; Tischler, Marc E. / Lysosomal proteolysis in distally or proximally denervated rat soleus muscle. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 1997 ; Vol. 273, No. 4 42-4.
@article{d604223defa34ab793b312660535a14b,
title = "Lysosomal proteolysis in distally or proximally denervated rat soleus muscle",
abstract = "We examined the mechanism of accelerated proteolysis in denervated rat soleus muscles. The soleus was denervated by severing either the tibial nerve (proximal, short stump) or sciatic nerve (distal, long stump) at 24, 48, 72, or 96 h before excision. Twenty-four hours after denervation, the extent of atrophy was similar for proximal and distal denervation, although lysosomal latency declined in both groups. After 48 and 72 h, denervation resulted in a decline in protein content, an increase in in vitro protein degradation, and a decline in lysosomal latency, all of which were greater in proximally denervated than in contralateral distally denervated muscles. These differences between acute responses of proximally and distally denervated muscles suggest the retention of some factor in the longer nerve stump that attenuates atrophy. After 96 h, total protein loss, protein degradation, and lysosomal latency were similar for proximal and distal denervation, suggesting the loss of axoplasmic flow from the long nerve stump.",
keywords = "Muscle atrophy, Neurotrophic factors, Sciatic nerve, Tibial nerve",
author = "Weinstein, {Randi B.} and Slentz, {Michael J.} and Kimberly Webster and Takeuchi, {Julie A.} and Tischler, {Marc E}",
year = "1997",
language = "English (US)",
volume = "273",
journal = "American Journal of Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "4 42-4",

}

TY - JOUR

T1 - Lysosomal proteolysis in distally or proximally denervated rat soleus muscle

AU - Weinstein, Randi B.

AU - Slentz, Michael J.

AU - Webster, Kimberly

AU - Takeuchi, Julie A.

AU - Tischler, Marc E

PY - 1997

Y1 - 1997

N2 - We examined the mechanism of accelerated proteolysis in denervated rat soleus muscles. The soleus was denervated by severing either the tibial nerve (proximal, short stump) or sciatic nerve (distal, long stump) at 24, 48, 72, or 96 h before excision. Twenty-four hours after denervation, the extent of atrophy was similar for proximal and distal denervation, although lysosomal latency declined in both groups. After 48 and 72 h, denervation resulted in a decline in protein content, an increase in in vitro protein degradation, and a decline in lysosomal latency, all of which were greater in proximally denervated than in contralateral distally denervated muscles. These differences between acute responses of proximally and distally denervated muscles suggest the retention of some factor in the longer nerve stump that attenuates atrophy. After 96 h, total protein loss, protein degradation, and lysosomal latency were similar for proximal and distal denervation, suggesting the loss of axoplasmic flow from the long nerve stump.

AB - We examined the mechanism of accelerated proteolysis in denervated rat soleus muscles. The soleus was denervated by severing either the tibial nerve (proximal, short stump) or sciatic nerve (distal, long stump) at 24, 48, 72, or 96 h before excision. Twenty-four hours after denervation, the extent of atrophy was similar for proximal and distal denervation, although lysosomal latency declined in both groups. After 48 and 72 h, denervation resulted in a decline in protein content, an increase in in vitro protein degradation, and a decline in lysosomal latency, all of which were greater in proximally denervated than in contralateral distally denervated muscles. These differences between acute responses of proximally and distally denervated muscles suggest the retention of some factor in the longer nerve stump that attenuates atrophy. After 96 h, total protein loss, protein degradation, and lysosomal latency were similar for proximal and distal denervation, suggesting the loss of axoplasmic flow from the long nerve stump.

KW - Muscle atrophy

KW - Neurotrophic factors

KW - Sciatic nerve

KW - Tibial nerve

UR - http://www.scopus.com/inward/record.url?scp=0030657574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030657574&partnerID=8YFLogxK

M3 - Article

VL - 273

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6143

IS - 4 42-4

ER -