Lysosomal proteolysis in distally or proximally denervated rat soleus muscle

Randi B. Weinstein, Michael J. Slentz, Kimberly Webster, Julie A. Takeuchi, Marc E. Tischler

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We examined the mechanism of accelerated proteolysis in denervated rat soleus muscles. The soleus was denervated by severing either the tibial nerve (proximal, short stump) or sciatic nerve (distal, long stump) at 24, 48, 72, or 96 h before excision. Twenty-four hours after denervation, the extent of atrophy was similar for proximal and distal denervation, although lysosomal latency declined in both groups. After 48 and 72 h, denervation resulted in a decline in protein content, an increase in in vitro protein degradation, and a decline in lysosomal latency, all of which were greater in proximally denervated than in contralateral distally denervated muscles. These differences between acute responses of proximally and distally denervated muscles suggest the retention of some factor in the longer nerve stump that attenuates atrophy. After 96 h, total protein loss, protein degradation, and lysosomal latency were similar for proximal and distal denervation, suggesting the loss of axoplasmic flow from the long nerve stump.

Original languageEnglish (US)
Pages (from-to)R1562-R1565
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume273
Issue number4 42-4
StatePublished - Nov 17 1997

    Fingerprint

Keywords

  • Muscle atrophy
  • Neurotrophic factors
  • Sciatic nerve
  • Tibial nerve

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this