Macaca fascicularis papillomavirus type 1: A non-human primate betapapillomavirus causing rapidly progressive hand and foot papillomatosis

Joongho Joh, Kelly Hopper, Koenraad Van Doorslaer, John P. Sundberg, Alfred B. Jenson, Shin Je Ghim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Papillomaviruses (PVs) are a group of small, non-enveloped DNA viruses that cause mucosal or cutaneous neoplasia in a variety of animals. Whilst most papillomas will regress spontaneously, some may persist or undergo malignant transformation. In this study, aggressive, persistent and extensive warts were observed on the hands and feet of a cynomolgus macaque (Macaca fascicularis). The presence of PV in the wart biopsies was identified by immunohistochemistry and PCR amplification of PV DNA. The genomic DNA of this PV was cloned and sequenced, and the PV was designated M. fascicularis papillomavirus type 1 (MfPV-1). Its genome was 7588 bp in length and the organization of its putative open reading frames (E1, E2, E6, E7, L1, L2 and E4) was similar to that of other PVs. MfPV-1 had a short non-coding region (NCR) of 412 bp. Molecular analysis of MfPV-1 genomic DNA classified it into the genus Betapapillomavirus, to which all epidermodysplasia verruciformis (EV)-type PVs belong. Diseases caused by PVs of the genus Betapapillomavirus are usually associated with natural or iatrogenic immunosuppression. The genomic characterization performed in this study showed that MfPV-1 clustered within the genus Betapapillomavirus and also contained EV-type-specific motifs in its NCR. Further characterization of this virus and its host interactions may allow us to develop a non-human primate model for human betapapillomaviruses, a genus populated by human PV types causing EV.

Original languageEnglish (US)
Pages (from-to)987-994
Number of pages8
JournalJournal of General Virology
Volume90
Issue number4
DOIs
StatePublished - Jun 29 2009
Externally publishedYes

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Betapapillomavirus
Macaca fascicularis
Papilloma
Primates
Epidermodysplasia Verruciformis
Foot
Hand
Warts
DNA
DNA Viruses
Macaca
Immunosuppression
Open Reading Frames
Immunohistochemistry
Genome
Viruses
Biopsy
Polymerase Chain Reaction
Skin

ASJC Scopus subject areas

  • Virology

Cite this

Macaca fascicularis papillomavirus type 1 : A non-human primate betapapillomavirus causing rapidly progressive hand and foot papillomatosis. / Joh, Joongho; Hopper, Kelly; Van Doorslaer, Koenraad; Sundberg, John P.; Jenson, Alfred B.; Ghim, Shin Je.

In: Journal of General Virology, Vol. 90, No. 4, 29.06.2009, p. 987-994.

Research output: Contribution to journalArticle

Joh, Joongho ; Hopper, Kelly ; Van Doorslaer, Koenraad ; Sundberg, John P. ; Jenson, Alfred B. ; Ghim, Shin Je. / Macaca fascicularis papillomavirus type 1 : A non-human primate betapapillomavirus causing rapidly progressive hand and foot papillomatosis. In: Journal of General Virology. 2009 ; Vol. 90, No. 4. pp. 987-994.
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