Magnaporthe grisea infection triggers RNA variation and antisense transcript expression in rice

Malali Gowda, R. C. Venu, Huameng Li, Chatchawan Jantasuriyarat, Songbiao Chen, Maria Bellizzi, Vishal Pampanwar, Hyeran Kim, Ralph A. Dean, Eric Stahlberg, Rod A Wing, Cari Soderlund, Guo Liang Wang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Rice blast disease, caused by the fungal pathogen Magnaporthe grisea, is an excellent model system to study plant-fungal interactions and host defense responses. In this study, comprehensive analysis of the rice (Oryza sativa) transcriptome after M. grisea infection was conducted using robust-long serial analysis of gene expression. A total of 83,382 distinct 21-bp robust-long serial analysis of gene expression tags were identified from 627,262 individual tags isolated from the resistant (R), susceptible (S), and control (C) libraries. Sequence analysis revealed that the tags in the R and S libraries had a significant reduced matching rate to the rice genomic and expressed sequences in comparison to the C library. The high level of one-nucleotide mismatches of the R and S library tags was due to nucleotide conversions. The A-to-G and U-to-C nucleotide conversions were the most predominant types, which were induced in the M. grisea-infected plants. Reverse transcription-polymerase chain reaction analysis showed that expression of the adenine deaminase and cytidine deaminase genes was highly induced after inoculation. In addition, many antisense transcripts were induced in infected plants and expression of four antisense transcripts was confirmed by strand-specific reverse transcription-polymerase chain reaction. These results demonstrate that there is a series of dynamic and complex transcript modifications and changes in the rice transcriptome at the M. grisea early infection stages.

Original languageEnglish (US)
Pages (from-to)524-533
Number of pages10
JournalPlant Physiology
Volume144
Issue number1
DOIs
StatePublished - May 2007

Fingerprint

Magnaporthe
Antisense RNA
Magnaporthe grisea
Libraries
RNA
rice
blast disease
nucleotides
Infection
transcriptome
infection
Nucleotides
adenine deaminase
Transcriptome
reverse transcriptase polymerase chain reaction
Reverse Transcription
gene expression
Cytidine Deaminase
Gene Expression
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Plant Science

Cite this

Gowda, M., Venu, R. C., Li, H., Jantasuriyarat, C., Chen, S., Bellizzi, M., ... Wang, G. L. (2007). Magnaporthe grisea infection triggers RNA variation and antisense transcript expression in rice. Plant Physiology, 144(1), 524-533. https://doi.org/10.1104/pp.107.095653

Magnaporthe grisea infection triggers RNA variation and antisense transcript expression in rice. / Gowda, Malali; Venu, R. C.; Li, Huameng; Jantasuriyarat, Chatchawan; Chen, Songbiao; Bellizzi, Maria; Pampanwar, Vishal; Kim, Hyeran; Dean, Ralph A.; Stahlberg, Eric; Wing, Rod A; Soderlund, Cari; Wang, Guo Liang.

In: Plant Physiology, Vol. 144, No. 1, 05.2007, p. 524-533.

Research output: Contribution to journalArticle

Gowda, M, Venu, RC, Li, H, Jantasuriyarat, C, Chen, S, Bellizzi, M, Pampanwar, V, Kim, H, Dean, RA, Stahlberg, E, Wing, RA, Soderlund, C & Wang, GL 2007, 'Magnaporthe grisea infection triggers RNA variation and antisense transcript expression in rice', Plant Physiology, vol. 144, no. 1, pp. 524-533. https://doi.org/10.1104/pp.107.095653
Gowda, Malali ; Venu, R. C. ; Li, Huameng ; Jantasuriyarat, Chatchawan ; Chen, Songbiao ; Bellizzi, Maria ; Pampanwar, Vishal ; Kim, Hyeran ; Dean, Ralph A. ; Stahlberg, Eric ; Wing, Rod A ; Soderlund, Cari ; Wang, Guo Liang. / Magnaporthe grisea infection triggers RNA variation and antisense transcript expression in rice. In: Plant Physiology. 2007 ; Vol. 144, No. 1. pp. 524-533.
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