Magnetic alignment and orientational order of dipalmitoylphosphatidylcholine bilayers containing palmitoyllsyophosphatidylcholine

Mikael Jansson, Robin L. Thurmond, Theodore P. Trouard, Michael F. Brown

Research output: Contribution to journalArticle

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Abstract

Mixed bilayers of 1-palmitoyl-sn-glycero-3-phosphocholine (palmitoyllysophosphatidylcholine; PaLPC) and 1,2-dipalmitoyl-sn- glycero-3-phosphocholine (dipalmitoyl phosphatidylcholine; DPPC) have been investigated by 2H-NMR and 31P-NMR spectroscopy. Binary phospholipid mixtures were studied in which the acyl chains of one or the other component were perdeuterated. At temperatures below the main order-disorder phase transition, the mixed PaLPC/DPPC bilayers appear to coexist with PaLPC micelles. The micelles disappear at temperatures above the phase transition, where mixed bilayers in the liquid-crystalline state are formed. The orientational order of the alkyl chains of the PaLPC component is essentially identical to that of the DPPC component in the mixed bilayers, both in the low temperature and liquid-crystalline phase. However, the presence of PaLPC perturbs the segmental ordering of DPPC as compared to the pure system. The order is increased in the low-temperature phase, where effective diffusion of the chains about their long axes occurs, but is decreased in the liquid-crystalline phase compared to pure DPPC bilayers. The mixed liquid-crystalline bilayers orient preferentially with their director axes perpendicular to the magnetic field. This alignment is easily observed in 31P- and 2H-NMR spectra, where the intensity of the perpendicular edges of the lineshapes is pronounced. One possible explanation of the magnetic alignment involves alteration of the curvature free energy of the DPPC bilayer due to incorporation of PaLPC in the mixed membranes.

Original languageEnglish (US)
Pages (from-to)157-170
Number of pages14
JournalChemistry and Physics of Lipids
Volume54
Issue number3-4
DOIs
Publication statusPublished - 1990

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Keywords

  • deuterium NMR spectroscopy
  • DPPC
  • lysolecithin
  • magnetic alignment

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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