Mammary uptake, portal-drained visceral flux, and hepatic metabolism of free and peptide-bound amino acids in cows fed steam-flaked or dry-rolled sorghum grain diets

H. Tagari, K. Webb, B. Theurer, T. Huber, D. DeYoung, S Peder Cuneo, J. E P Santos, J. Simas, M. Sadik, A. Alio, O. Lozano, A. Delgado-Elorduy, L. Nussio, C. M M Bittar, F. Santos

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Our objectives were to measure net fluxes of free AA (FAA) and peptide-bound AA (PBAA) across portal-drained viscera, liver, splanchnic tissues, and mammary tissues, and milk AA output of lactating Holstein cows (n = 8, 86 ± 8 d in milk). Cows were fed an alfalfa-based total mixed ration containing 40% steam-flaked (SFS) or dry-rolled (DRS) sorghum grain. The total mixed rations were offered at 12-h intervals in a crossover design. Blood samples were obtained from indwelling catheters in portal, hepatic, and mammary veins and from mesenteric or costoabdominal arteries every 2 h from each cow and diet. Intake of dry matter was 17.9 and 18.6 kg/d of the SFS and DRS diets, respectively, but dropped to 16.3 kg/d for cows fed the SFS diet in the last 3 experimental days, sampling day included. Milk and milk crude protein yields (kg/12-h sampling) were 13.85 vs. 13.25 and 0.425 vs. 0.396 for cows fed SFS or DRS, respectively, and were not affected by the considerable drop in dry matter intake of cows fed the SFS diet during the last 3 experimental days. The portal-drained visceral flux of total essential FAA was 417 and 442 g/12 h (SEM 63) in cows fed SFS and DRS, respectively. However, the portal-drained visceral flux of 7 essential PBAA out of the 9 determined was numerically greater in cows fed the SFS diet, and total essential PBAA in that treatment was 77.4 ± 22.2 compared with 35.4 ± 50.2 g/12 h for cows fed the DRS diet. This phenomenon was again observed in a greater total splanchnic flux (FAA + PBAA) of 462 and 371 g/12 h in SFS- and DRS-fed cows, respectively. Mammary uptake of essential AA from both pools (free and peptide bound), and recovery of essential AA in milk, was again numerically higher in SFS-fed cows. In addition to FAA, quantifying the contribution of PBAA may improve our understanding of tissue use of AA substrates, and this may ultimately lead to improved diet formulations with respect to intestinal absorption and mammary uptake of AA.

Original languageEnglish (US)
Pages (from-to)679-697
Number of pages19
JournalJournal of Dairy Science
Volume91
Issue number2
DOIs
StatePublished - Feb 2008

Fingerprint

Sorghum
Steam
grain sorghum
steam
breasts
Breast
peptides
Diet
uptake mechanisms
Amino Acids
cows
Peptides
liver
amino acids
metabolism
Liver
Viscera
diet
Milk
milk

Keywords

  • Amino acid
  • Dairy cow
  • Flaked sorghum
  • Peptide

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Food Science

Cite this

Mammary uptake, portal-drained visceral flux, and hepatic metabolism of free and peptide-bound amino acids in cows fed steam-flaked or dry-rolled sorghum grain diets. / Tagari, H.; Webb, K.; Theurer, B.; Huber, T.; DeYoung, D.; Cuneo, S Peder; Santos, J. E P; Simas, J.; Sadik, M.; Alio, A.; Lozano, O.; Delgado-Elorduy, A.; Nussio, L.; Bittar, C. M M; Santos, F.

In: Journal of Dairy Science, Vol. 91, No. 2, 02.2008, p. 679-697.

Research output: Contribution to journalArticle

Tagari, H, Webb, K, Theurer, B, Huber, T, DeYoung, D, Cuneo, SP, Santos, JEP, Simas, J, Sadik, M, Alio, A, Lozano, O, Delgado-Elorduy, A, Nussio, L, Bittar, CMM & Santos, F 2008, 'Mammary uptake, portal-drained visceral flux, and hepatic metabolism of free and peptide-bound amino acids in cows fed steam-flaked or dry-rolled sorghum grain diets', Journal of Dairy Science, vol. 91, no. 2, pp. 679-697. https://doi.org/10.3168/jds.2007-0629
Tagari, H. ; Webb, K. ; Theurer, B. ; Huber, T. ; DeYoung, D. ; Cuneo, S Peder ; Santos, J. E P ; Simas, J. ; Sadik, M. ; Alio, A. ; Lozano, O. ; Delgado-Elorduy, A. ; Nussio, L. ; Bittar, C. M M ; Santos, F. / Mammary uptake, portal-drained visceral flux, and hepatic metabolism of free and peptide-bound amino acids in cows fed steam-flaked or dry-rolled sorghum grain diets. In: Journal of Dairy Science. 2008 ; Vol. 91, No. 2. pp. 679-697.
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abstract = "Our objectives were to measure net fluxes of free AA (FAA) and peptide-bound AA (PBAA) across portal-drained viscera, liver, splanchnic tissues, and mammary tissues, and milk AA output of lactating Holstein cows (n = 8, 86 ± 8 d in milk). Cows were fed an alfalfa-based total mixed ration containing 40{\%} steam-flaked (SFS) or dry-rolled (DRS) sorghum grain. The total mixed rations were offered at 12-h intervals in a crossover design. Blood samples were obtained from indwelling catheters in portal, hepatic, and mammary veins and from mesenteric or costoabdominal arteries every 2 h from each cow and diet. Intake of dry matter was 17.9 and 18.6 kg/d of the SFS and DRS diets, respectively, but dropped to 16.3 kg/d for cows fed the SFS diet in the last 3 experimental days, sampling day included. Milk and milk crude protein yields (kg/12-h sampling) were 13.85 vs. 13.25 and 0.425 vs. 0.396 for cows fed SFS or DRS, respectively, and were not affected by the considerable drop in dry matter intake of cows fed the SFS diet during the last 3 experimental days. The portal-drained visceral flux of total essential FAA was 417 and 442 g/12 h (SEM 63) in cows fed SFS and DRS, respectively. However, the portal-drained visceral flux of 7 essential PBAA out of the 9 determined was numerically greater in cows fed the SFS diet, and total essential PBAA in that treatment was 77.4 ± 22.2 compared with 35.4 ± 50.2 g/12 h for cows fed the DRS diet. This phenomenon was again observed in a greater total splanchnic flux (FAA + PBAA) of 462 and 371 g/12 h in SFS- and DRS-fed cows, respectively. Mammary uptake of essential AA from both pools (free and peptide bound), and recovery of essential AA in milk, was again numerically higher in SFS-fed cows. In addition to FAA, quantifying the contribution of PBAA may improve our understanding of tissue use of AA substrates, and this may ultimately lead to improved diet formulations with respect to intestinal absorption and mammary uptake of AA.",
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AU - Tagari, H.

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AU - Theurer, B.

AU - Huber, T.

AU - DeYoung, D.

AU - Cuneo, S Peder

AU - Santos, J. E P

AU - Simas, J.

AU - Sadik, M.

AU - Alio, A.

AU - Lozano, O.

AU - Delgado-Elorduy, A.

AU - Nussio, L.

AU - Bittar, C. M M

AU - Santos, F.

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N2 - Our objectives were to measure net fluxes of free AA (FAA) and peptide-bound AA (PBAA) across portal-drained viscera, liver, splanchnic tissues, and mammary tissues, and milk AA output of lactating Holstein cows (n = 8, 86 ± 8 d in milk). Cows were fed an alfalfa-based total mixed ration containing 40% steam-flaked (SFS) or dry-rolled (DRS) sorghum grain. The total mixed rations were offered at 12-h intervals in a crossover design. Blood samples were obtained from indwelling catheters in portal, hepatic, and mammary veins and from mesenteric or costoabdominal arteries every 2 h from each cow and diet. Intake of dry matter was 17.9 and 18.6 kg/d of the SFS and DRS diets, respectively, but dropped to 16.3 kg/d for cows fed the SFS diet in the last 3 experimental days, sampling day included. Milk and milk crude protein yields (kg/12-h sampling) were 13.85 vs. 13.25 and 0.425 vs. 0.396 for cows fed SFS or DRS, respectively, and were not affected by the considerable drop in dry matter intake of cows fed the SFS diet during the last 3 experimental days. The portal-drained visceral flux of total essential FAA was 417 and 442 g/12 h (SEM 63) in cows fed SFS and DRS, respectively. However, the portal-drained visceral flux of 7 essential PBAA out of the 9 determined was numerically greater in cows fed the SFS diet, and total essential PBAA in that treatment was 77.4 ± 22.2 compared with 35.4 ± 50.2 g/12 h for cows fed the DRS diet. This phenomenon was again observed in a greater total splanchnic flux (FAA + PBAA) of 462 and 371 g/12 h in SFS- and DRS-fed cows, respectively. Mammary uptake of essential AA from both pools (free and peptide bound), and recovery of essential AA in milk, was again numerically higher in SFS-fed cows. In addition to FAA, quantifying the contribution of PBAA may improve our understanding of tissue use of AA substrates, and this may ultimately lead to improved diet formulations with respect to intestinal absorption and mammary uptake of AA.

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KW - Amino acid

KW - Dairy cow

KW - Flaked sorghum

KW - Peptide

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