Mannitol opening of the blood-brain barrier: Regional variation in the permeability of sucrose, but not 86Rb+ or albumin

Rachel C. Brown, Richard D. Egleton, Thomas P Davis

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Clinically, infusion of hyperosmolar solutions is used to enhance chemotherapeutic drug penetration of the blood-brain barrier (BBB) in patients with malignant brain tumors or metastases. We examined the effect of hyperosmolar BBB disruption on brain permeability of three compounds, 86Rb+, a marker for K+ permeability and transport, [14C]sucrose and Evans blue albumin, using a rat in situ perfusion model. 86Rb+ and [14C]sucrose had increased permeability 20 min after BBB disruption with 1.6 M mannitol. There was no change in Evans blue albumin permeability. Only [14C]sucrose showed regional variation in permeability after mannitol-induced BBB disruption, with the cortex and midbrain having higher sucrose permeability then either the cerebellum or brainstem. These data suggest that the clinical efficacy of hyperosmolar disruption therapy in conjunction with chemotherapeutic agents, of a similar molecular weight to sucrose, may be affected by the location of the tumor within the brain.

Original languageEnglish (US)
Pages (from-to)221-227
Number of pages7
JournalBrain Research
Volume1014
Issue number1-2
DOIs
StatePublished - Jul 16 2004

Fingerprint

Mannitol
Blood-Brain Barrier
Sucrose
Albumins
Permeability
Evans Blue
Brain Neoplasms
Mesencephalon
Cerebellum
Brain Stem
Perfusion
Molecular Weight
Neoplasm Metastasis
Brain
Pharmaceutical Preparations

Keywords

  • Disorders of the nervous systems
  • Hyperosmolar mannitol
  • In situ perfusion
  • Neuro-oncology
  • Permeability
  • Tight junction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mannitol opening of the blood-brain barrier : Regional variation in the permeability of sucrose, but not 86Rb+ or albumin. / Brown, Rachel C.; Egleton, Richard D.; Davis, Thomas P.

In: Brain Research, Vol. 1014, No. 1-2, 16.07.2004, p. 221-227.

Research output: Contribution to journalArticle

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