Mannose-binding lectin serum levels are low in persons with clinically active coccidioidomycosis

Neil M. Ampel, Sara O. Dionne, Andrea Giblin, Abigail B. Podany, John N Galgiani

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Mannose-binding lectin (MBL) is a circulating collectin that is part of the innate immune response. We explored the serum levels of MBL in persons with different forms of coccidioidomycosis. Methods: Serum MBL was measured by ELISA from samples obtained from healthy donors with immunity to Coccidioides, and those with various forms of active coccidioidomycosis. Blood cell specimens from a subgroup of subjects with active coccidioidomycosis were examined for single nucleotide polymorphisms of the MBL gene and promoter regions. Results: The control group comprised 29 healthy immune subjects. Patient groups with active coccidioidomycosis consisted of 20 patients with symptomatic primary pulmonary coccidioidomycosis, 26 with non-meningeal disseminated coccidioidomycosis, and nine with coccidioidal meningitis. The group with active coccidioidomycosis was significantly older and more likely to be male than the control group (for both, P < 0.001). The mean ± SEM level of serum MBL in the healthy controls was 169.4 ± 28.6 ng/ml, significantly higher than the 79.2 ± 10.9 ng/ml for all active groups (P < 0.001). Moreover, the active coccidioidomycosis group was significantly more likely to have serum MBL level 70 ng/ml compared to the control group (P = 0.001). Genetic analysis in 27 subjects with active coccidioidomycosis revealed marked variation based on race and ethnicity. Among a subgroup of 10 white, non-hispanic men with active coccidioidomycosis, there was a significant association between the H and P haplotypes and MBL levels 70 ng/ml (P < 0.036 and P < 0.035, respectively). Conclusions: These data suggest that there is an association between low serum MBL levels and symptomatic coccidioidomycosis.

Original languageEnglish (US)
Pages (from-to)173-180
Number of pages8
JournalMycopathologia
Volume167
Issue number4
DOIs
StatePublished - Apr 2009

Fingerprint

coccidioidomycosis
Coccidioidomycosis
Mannose-Binding Lectin
mannose
lectins
Serum
Control Groups
Coccidioides
Collectins
meningitis
blood cells
nationalities and ethnic groups
Meningitis
Innate Immunity
Genetic Promoter Regions
Haplotypes
single nucleotide polymorphism
Single Nucleotide Polymorphism
genetic techniques and protocols
Immunity

Keywords

  • Coccidioides
  • Coccidioidomycosis
  • Collectins
  • Human
  • Innate immunity
  • Mannose binding lectin

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Applied Microbiology and Biotechnology
  • Microbiology
  • veterinary (miscalleneous)

Cite this

Mannose-binding lectin serum levels are low in persons with clinically active coccidioidomycosis. / Ampel, Neil M.; Dionne, Sara O.; Giblin, Andrea; Podany, Abigail B.; Galgiani, John N.

In: Mycopathologia, Vol. 167, No. 4, 04.2009, p. 173-180.

Research output: Contribution to journalArticle

Ampel, Neil M. ; Dionne, Sara O. ; Giblin, Andrea ; Podany, Abigail B. ; Galgiani, John N. / Mannose-binding lectin serum levels are low in persons with clinically active coccidioidomycosis. In: Mycopathologia. 2009 ; Vol. 167, No. 4. pp. 173-180.
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AU - Dionne, Sara O.

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AU - Galgiani, John N

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