Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley

A. J. Castro, F. Capettini, A. E. Corey, T. Filichkina, P. M. Hayes, A. Kleinhofs, David A Kudrna, K. Richardson, S. Sandoval-Islas, C. Rossi, H. Vivar

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The identification and location of sources of genetic resistance to plant diseases are important contributions to the development of resistant varieties. The combination of different sources and types of resistance in the same genotype should assist in the development of durably resistant varieties. Using a doubled haploid (DH), mapping population of barley, we mapped a qualitative resistance gene (Rpsx) to barley stripe rust in the accession CI10587 (PI 243183) to the long arm of chromosome 1(7H). We combined the Rpsx gene, through a series of crosses, with three mapped and validated barley stripe rust resistance QTL alleles located on chromosomes 4(4H) (QTL4), 5(1H) (QTL5), and 7(5H) (QTL7). Three different barley DH populations were developed from these crosses, two combining Rpsx with QTL4 and QTL7, and the third combining Rpsx with QTL5. Disease severity testing in four environments and QTL mapping analyses confirmed the effects and locations of Rpsx, QTL4, and QTL5, thereby validating the original estimates of QTL location and effect. QTL alleles on chromosomes 4(4H) and 5(1H) were effective in decreasing disease severity in the absence of the resistance allele at Rpsx. Quantitative resistance effects were mainly additive, although magnitude interactions were detected. Our results indicate that combining qualitative and quantitative resistance in the same genotype is feasible. However, the durability of such resistance pyramids will require challenge from virulent isolates, which currently are not reported in North America.

Original languageEnglish (US)
Pages (from-to)922-930
Number of pages9
JournalTheoretical And Applied Genetics
Volume107
Issue number5
DOIs
StatePublished - Sep 1 2003

Fingerprint

Hordeum
quantitative trait loci
barley
Chromosomes, Human, Pair 4
Haploidy
Alleles
doubled haploids
alleles
chromosomes
disease severity
Genotype
Plant Diseases
Chromosomes, Human, Pair 5
genotype
Chromosomes, Human, Pair 1
genetic resistance
durability
plant diseases and disorders
North America
Population

Keywords

  • Barley
  • QTL
  • Quantitative resistance
  • Stripe rust

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

Castro, A. J., Capettini, F., Corey, A. E., Filichkina, T., Hayes, P. M., Kleinhofs, A., ... Vivar, H. (2003). Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley. Theoretical And Applied Genetics, 107(5), 922-930. https://doi.org/10.1007/s00122-003-1329-6

Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley. / Castro, A. J.; Capettini, F.; Corey, A. E.; Filichkina, T.; Hayes, P. M.; Kleinhofs, A.; Kudrna, David A; Richardson, K.; Sandoval-Islas, S.; Rossi, C.; Vivar, H.

In: Theoretical And Applied Genetics, Vol. 107, No. 5, 01.09.2003, p. 922-930.

Research output: Contribution to journalArticle

Castro, AJ, Capettini, F, Corey, AE, Filichkina, T, Hayes, PM, Kleinhofs, A, Kudrna, DA, Richardson, K, Sandoval-Islas, S, Rossi, C & Vivar, H 2003, 'Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley', Theoretical And Applied Genetics, vol. 107, no. 5, pp. 922-930. https://doi.org/10.1007/s00122-003-1329-6
Castro, A. J. ; Capettini, F. ; Corey, A. E. ; Filichkina, T. ; Hayes, P. M. ; Kleinhofs, A. ; Kudrna, David A ; Richardson, K. ; Sandoval-Islas, S. ; Rossi, C. ; Vivar, H. / Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley. In: Theoretical And Applied Genetics. 2003 ; Vol. 107, No. 5. pp. 922-930.
@article{f6d2253f8a794d49b353de034e62b8a4,
title = "Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley",
abstract = "The identification and location of sources of genetic resistance to plant diseases are important contributions to the development of resistant varieties. The combination of different sources and types of resistance in the same genotype should assist in the development of durably resistant varieties. Using a doubled haploid (DH), mapping population of barley, we mapped a qualitative resistance gene (Rpsx) to barley stripe rust in the accession CI10587 (PI 243183) to the long arm of chromosome 1(7H). We combined the Rpsx gene, through a series of crosses, with three mapped and validated barley stripe rust resistance QTL alleles located on chromosomes 4(4H) (QTL4), 5(1H) (QTL5), and 7(5H) (QTL7). Three different barley DH populations were developed from these crosses, two combining Rpsx with QTL4 and QTL7, and the third combining Rpsx with QTL5. Disease severity testing in four environments and QTL mapping analyses confirmed the effects and locations of Rpsx, QTL4, and QTL5, thereby validating the original estimates of QTL location and effect. QTL alleles on chromosomes 4(4H) and 5(1H) were effective in decreasing disease severity in the absence of the resistance allele at Rpsx. Quantitative resistance effects were mainly additive, although magnitude interactions were detected. Our results indicate that combining qualitative and quantitative resistance in the same genotype is feasible. However, the durability of such resistance pyramids will require challenge from virulent isolates, which currently are not reported in North America.",
keywords = "Barley, QTL, Quantitative resistance, Stripe rust",
author = "Castro, {A. J.} and F. Capettini and Corey, {A. E.} and T. Filichkina and Hayes, {P. M.} and A. Kleinhofs and Kudrna, {David A} and K. Richardson and S. Sandoval-Islas and C. Rossi and H. Vivar",
year = "2003",
month = "9",
day = "1",
doi = "10.1007/s00122-003-1329-6",
language = "English (US)",
volume = "107",
pages = "922--930",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley

AU - Castro, A. J.

AU - Capettini, F.

AU - Corey, A. E.

AU - Filichkina, T.

AU - Hayes, P. M.

AU - Kleinhofs, A.

AU - Kudrna, David A

AU - Richardson, K.

AU - Sandoval-Islas, S.

AU - Rossi, C.

AU - Vivar, H.

PY - 2003/9/1

Y1 - 2003/9/1

N2 - The identification and location of sources of genetic resistance to plant diseases are important contributions to the development of resistant varieties. The combination of different sources and types of resistance in the same genotype should assist in the development of durably resistant varieties. Using a doubled haploid (DH), mapping population of barley, we mapped a qualitative resistance gene (Rpsx) to barley stripe rust in the accession CI10587 (PI 243183) to the long arm of chromosome 1(7H). We combined the Rpsx gene, through a series of crosses, with three mapped and validated barley stripe rust resistance QTL alleles located on chromosomes 4(4H) (QTL4), 5(1H) (QTL5), and 7(5H) (QTL7). Three different barley DH populations were developed from these crosses, two combining Rpsx with QTL4 and QTL7, and the third combining Rpsx with QTL5. Disease severity testing in four environments and QTL mapping analyses confirmed the effects and locations of Rpsx, QTL4, and QTL5, thereby validating the original estimates of QTL location and effect. QTL alleles on chromosomes 4(4H) and 5(1H) were effective in decreasing disease severity in the absence of the resistance allele at Rpsx. Quantitative resistance effects were mainly additive, although magnitude interactions were detected. Our results indicate that combining qualitative and quantitative resistance in the same genotype is feasible. However, the durability of such resistance pyramids will require challenge from virulent isolates, which currently are not reported in North America.

AB - The identification and location of sources of genetic resistance to plant diseases are important contributions to the development of resistant varieties. The combination of different sources and types of resistance in the same genotype should assist in the development of durably resistant varieties. Using a doubled haploid (DH), mapping population of barley, we mapped a qualitative resistance gene (Rpsx) to barley stripe rust in the accession CI10587 (PI 243183) to the long arm of chromosome 1(7H). We combined the Rpsx gene, through a series of crosses, with three mapped and validated barley stripe rust resistance QTL alleles located on chromosomes 4(4H) (QTL4), 5(1H) (QTL5), and 7(5H) (QTL7). Three different barley DH populations were developed from these crosses, two combining Rpsx with QTL4 and QTL7, and the third combining Rpsx with QTL5. Disease severity testing in four environments and QTL mapping analyses confirmed the effects and locations of Rpsx, QTL4, and QTL5, thereby validating the original estimates of QTL location and effect. QTL alleles on chromosomes 4(4H) and 5(1H) were effective in decreasing disease severity in the absence of the resistance allele at Rpsx. Quantitative resistance effects were mainly additive, although magnitude interactions were detected. Our results indicate that combining qualitative and quantitative resistance in the same genotype is feasible. However, the durability of such resistance pyramids will require challenge from virulent isolates, which currently are not reported in North America.

KW - Barley

KW - QTL

KW - Quantitative resistance

KW - Stripe rust

UR - http://www.scopus.com/inward/record.url?scp=0141976685&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141976685&partnerID=8YFLogxK

U2 - 10.1007/s00122-003-1329-6

DO - 10.1007/s00122-003-1329-6

M3 - Article

C2 - 12845434

AN - SCOPUS:0141976685

VL - 107

SP - 922

EP - 930

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 5

ER -