Mapping CRMP3 domains involved in dendrite morphogenesis and voltage-gated calcium channel regulation

Tam T. Quach, Sarah M. Wilson, Veronique Rogemond, Naura Chounlamountri, Pappachan E. Kolattukudy, Stephanie Martinez, May Khanna, Marie Francoise Belin, Rajesh Khanna, Jerome Honnorat, Anne Marie Duchemin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Although hippocampal neurons are well-distinguished by the morphological characteristics of their dendrites and their structural plasticity, the mechanisms involved in regulating their neurite initiation, dendrite growth, network formation and remodeling are still largely unknown, in part because the key molecules involved remain elusive. Identifying new dendrite-active cues could uncover unknown molecular mechanisms that would add significant understanding to the field and possibly lead to the development of novel neuroprotective therapy because these neurons are impaired in many neuropsychiatric disorders. In our previous studies, we deleted the gene encoding CRMP3 in mice and identified the protein as a new endogenous signaling molecule that shapes diverse features of the hippocampal pyramidal dendrites without affecting axon morphology. We also found that CRMP3 protects dendrites against dystrophy induced by prion peptide PrP106-126. Here, we report that CRMP3 has a profound influence on neurite initiation and dendrite growth of hippocampal neurons in vitro. Our deletional mapping revealed that the C-terminus of CRMP3 probably harbors its dendritogenic capacity and supports an active transport mechanism. By contrast, overexpression of the C-terminal truncated CRMP3 phenocopied the effect of CRMP3 gene deletion with inhibition of neurite initiation or decrease in dendrite complexity, depending on the stage of cell development. In addition, this mutant inhibited the activity of CRMP3, in a similar manner to siRNA. Voltage-gated calcium channel inhibitors prevented CRMP3-induced dendritic growth and somatic Ca2+ influx in CRMP3-overexpressing neurons was augmented largely via L-type channels. These results support a link between CRMP3-mediated Ca2+ influx and CRMP3-mediated dendritic growth in hippocampal neurons.

Original languageEnglish (US)
Pages (from-to)4262-4273
Number of pages12
JournalJournal of Cell Science
Volume126
Issue number18
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Calcium Channels
Dendrites
Morphogenesis
Neurons
Neurites
Growth
Active Biological Transport
Prions
Gene Deletion
Small Interfering RNA
Cues
Axons
Peptides
Genes
Proteins

Keywords

  • Ca flux
  • CRMP3
  • Dendrite morphogenesis
  • Hippocampal neurons
  • Lamellipodia
  • Neurite initiation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Quach, T. T., Wilson, S. M., Rogemond, V., Chounlamountri, N., Kolattukudy, P. E., Martinez, S., ... Duchemin, A. M. (2013). Mapping CRMP3 domains involved in dendrite morphogenesis and voltage-gated calcium channel regulation. Journal of Cell Science, 126(18), 4262-4273. https://doi.org/10.1242/jcs.131409

Mapping CRMP3 domains involved in dendrite morphogenesis and voltage-gated calcium channel regulation. / Quach, Tam T.; Wilson, Sarah M.; Rogemond, Veronique; Chounlamountri, Naura; Kolattukudy, Pappachan E.; Martinez, Stephanie; Khanna, May; Belin, Marie Francoise; Khanna, Rajesh; Honnorat, Jerome; Duchemin, Anne Marie.

In: Journal of Cell Science, Vol. 126, No. 18, 2013, p. 4262-4273.

Research output: Contribution to journalArticle

Quach, TT, Wilson, SM, Rogemond, V, Chounlamountri, N, Kolattukudy, PE, Martinez, S, Khanna, M, Belin, MF, Khanna, R, Honnorat, J & Duchemin, AM 2013, 'Mapping CRMP3 domains involved in dendrite morphogenesis and voltage-gated calcium channel regulation', Journal of Cell Science, vol. 126, no. 18, pp. 4262-4273. https://doi.org/10.1242/jcs.131409
Quach TT, Wilson SM, Rogemond V, Chounlamountri N, Kolattukudy PE, Martinez S et al. Mapping CRMP3 domains involved in dendrite morphogenesis and voltage-gated calcium channel regulation. Journal of Cell Science. 2013;126(18):4262-4273. https://doi.org/10.1242/jcs.131409
Quach, Tam T. ; Wilson, Sarah M. ; Rogemond, Veronique ; Chounlamountri, Naura ; Kolattukudy, Pappachan E. ; Martinez, Stephanie ; Khanna, May ; Belin, Marie Francoise ; Khanna, Rajesh ; Honnorat, Jerome ; Duchemin, Anne Marie. / Mapping CRMP3 domains involved in dendrite morphogenesis and voltage-gated calcium channel regulation. In: Journal of Cell Science. 2013 ; Vol. 126, No. 18. pp. 4262-4273.
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