Mapping of a Dde I RELP of the α2-Adrenoceptor Gene and the Correlation of Genotype with Blood Pressure in the F2 Rats of SHR-WKY Cross

Lu Sun, Shari McArdle, Melanie Chun, Dennis W. Wolff, Nancy Schulte, William A. Pettinger, John W Regan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To examine a pathogenetic role of α2-adrenoceptor (α2-AR) in genetically hypertensive rats, we compared the α2-AR gene of spontaneously hypertensive rats (SHR) and its normotensive control Wistar-Kyoto (WKY) rats by restriction fragment length polymorphism (RFLP) analysis and did a genetic cosegregation study. After screening with 52 restriction endonucleases (RE), an RFLP was found by digestion of the genomic DNA of SHR and WKY rat with Dde I RE and using a human platelet α2-AR probe, the gene (ADRA2R) which is localized in chromosome 10. Restriction mapping analysis revealed that the RFLP is located in the 5′ region of the α2-AR gene. This RFLP was used as a marker in a genetic cosegregation study to examine the correlation of genotype with blood pressure in F2 rats of SHR and WKY rat cross. The distribution of the genotypes based on the RFLP conformed to a 1:2:1 ratio in male F2 rats as expected for Mendelian inheritance, but not in female F2 rats. The number of female F2 rats with double SHR alleles was decreased about 68% from expected. Blood pressure of the male or female F2 rats homozygous for the SHR α2-adrenoceptor alleles was not significantly higher than the F2 rats heterozygous or homozygous for the WKY alleles with either indirect or direct blood pressure measurement. The additional finding was that males had higher blood pressure than females. This study ruled out the cosegregation of α2-adrenoceptor RFLP with higher blood pressure in the F2 rats of SHR and WKY cross. However, Since the abnormality of the renal α2-adrenoceptor in genetically hypertensive rats is one of altered regulation, and the RFLP we found is located in the 5′ region of the gene where functionally important sequences are located which regulate gene expression, this RFLP may still be of interest in genetically hypertensive rats. (Hypertens Res 1992; 15: 143-148).

Original languageEnglish (US)
Pages (from-to)143-148
Number of pages6
JournalHypertension Research
Volume15
DOIs
StatePublished - 1992

Fingerprint

Inbred SHR Rats
Adrenergic Receptors
Restriction Fragment Length Polymorphisms
Genotype
Blood Pressure
Genes
Inbred WKY Rats
Alleles
DNA Restriction Enzymes
Hypertension
Chromosomes, Human, Pair 10
Restriction Mapping
Digestion
Blood Platelets
Kidney
Gene Expression
DNA

Keywords

  • RFLP
  • SHR
  • α-adrenoceptor

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Mapping of a Dde I RELP of the α2-Adrenoceptor Gene and the Correlation of Genotype with Blood Pressure in the F2 Rats of SHR-WKY Cross. / Sun, Lu; McArdle, Shari; Chun, Melanie; Wolff, Dennis W.; Schulte, Nancy; Pettinger, William A.; Regan, John W.

In: Hypertension Research, Vol. 15, 1992, p. 143-148.

Research output: Contribution to journalArticle

Sun, Lu ; McArdle, Shari ; Chun, Melanie ; Wolff, Dennis W. ; Schulte, Nancy ; Pettinger, William A. ; Regan, John W. / Mapping of a Dde I RELP of the α2-Adrenoceptor Gene and the Correlation of Genotype with Blood Pressure in the F2 Rats of SHR-WKY Cross. In: Hypertension Research. 1992 ; Vol. 15. pp. 143-148.
@article{1cad4ead3cc347b29cd9639eeef9a1a2,
title = "Mapping of a Dde I RELP of the α2-Adrenoceptor Gene and the Correlation of Genotype with Blood Pressure in the F2 Rats of SHR-WKY Cross",
abstract = "To examine a pathogenetic role of α2-adrenoceptor (α2-AR) in genetically hypertensive rats, we compared the α2-AR gene of spontaneously hypertensive rats (SHR) and its normotensive control Wistar-Kyoto (WKY) rats by restriction fragment length polymorphism (RFLP) analysis and did a genetic cosegregation study. After screening with 52 restriction endonucleases (RE), an RFLP was found by digestion of the genomic DNA of SHR and WKY rat with Dde I RE and using a human platelet α2-AR probe, the gene (ADRA2R) which is localized in chromosome 10. Restriction mapping analysis revealed that the RFLP is located in the 5′ region of the α2-AR gene. This RFLP was used as a marker in a genetic cosegregation study to examine the correlation of genotype with blood pressure in F2 rats of SHR and WKY rat cross. The distribution of the genotypes based on the RFLP conformed to a 1:2:1 ratio in male F2 rats as expected for Mendelian inheritance, but not in female F2 rats. The number of female F2 rats with double SHR alleles was decreased about 68{\%} from expected. Blood pressure of the male or female F2 rats homozygous for the SHR α2-adrenoceptor alleles was not significantly higher than the F2 rats heterozygous or homozygous for the WKY alleles with either indirect or direct blood pressure measurement. The additional finding was that males had higher blood pressure than females. This study ruled out the cosegregation of α2-adrenoceptor RFLP with higher blood pressure in the F2 rats of SHR and WKY cross. However, Since the abnormality of the renal α2-adrenoceptor in genetically hypertensive rats is one of altered regulation, and the RFLP we found is located in the 5′ region of the gene where functionally important sequences are located which regulate gene expression, this RFLP may still be of interest in genetically hypertensive rats. (Hypertens Res 1992; 15: 143-148).",
keywords = "RFLP, SHR, α-adrenoceptor",
author = "Lu Sun and Shari McArdle and Melanie Chun and Wolff, {Dennis W.} and Nancy Schulte and Pettinger, {William A.} and Regan, {John W}",
year = "1992",
doi = "10.1291/hypres.15.143",
language = "English (US)",
volume = "15",
pages = "143--148",
journal = "Hypertension Research",
issn = "0916-9636",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Mapping of a Dde I RELP of the α2-Adrenoceptor Gene and the Correlation of Genotype with Blood Pressure in the F2 Rats of SHR-WKY Cross

AU - Sun, Lu

AU - McArdle, Shari

AU - Chun, Melanie

AU - Wolff, Dennis W.

AU - Schulte, Nancy

AU - Pettinger, William A.

AU - Regan, John W

PY - 1992

Y1 - 1992

N2 - To examine a pathogenetic role of α2-adrenoceptor (α2-AR) in genetically hypertensive rats, we compared the α2-AR gene of spontaneously hypertensive rats (SHR) and its normotensive control Wistar-Kyoto (WKY) rats by restriction fragment length polymorphism (RFLP) analysis and did a genetic cosegregation study. After screening with 52 restriction endonucleases (RE), an RFLP was found by digestion of the genomic DNA of SHR and WKY rat with Dde I RE and using a human platelet α2-AR probe, the gene (ADRA2R) which is localized in chromosome 10. Restriction mapping analysis revealed that the RFLP is located in the 5′ region of the α2-AR gene. This RFLP was used as a marker in a genetic cosegregation study to examine the correlation of genotype with blood pressure in F2 rats of SHR and WKY rat cross. The distribution of the genotypes based on the RFLP conformed to a 1:2:1 ratio in male F2 rats as expected for Mendelian inheritance, but not in female F2 rats. The number of female F2 rats with double SHR alleles was decreased about 68% from expected. Blood pressure of the male or female F2 rats homozygous for the SHR α2-adrenoceptor alleles was not significantly higher than the F2 rats heterozygous or homozygous for the WKY alleles with either indirect or direct blood pressure measurement. The additional finding was that males had higher blood pressure than females. This study ruled out the cosegregation of α2-adrenoceptor RFLP with higher blood pressure in the F2 rats of SHR and WKY cross. However, Since the abnormality of the renal α2-adrenoceptor in genetically hypertensive rats is one of altered regulation, and the RFLP we found is located in the 5′ region of the gene where functionally important sequences are located which regulate gene expression, this RFLP may still be of interest in genetically hypertensive rats. (Hypertens Res 1992; 15: 143-148).

AB - To examine a pathogenetic role of α2-adrenoceptor (α2-AR) in genetically hypertensive rats, we compared the α2-AR gene of spontaneously hypertensive rats (SHR) and its normotensive control Wistar-Kyoto (WKY) rats by restriction fragment length polymorphism (RFLP) analysis and did a genetic cosegregation study. After screening with 52 restriction endonucleases (RE), an RFLP was found by digestion of the genomic DNA of SHR and WKY rat with Dde I RE and using a human platelet α2-AR probe, the gene (ADRA2R) which is localized in chromosome 10. Restriction mapping analysis revealed that the RFLP is located in the 5′ region of the α2-AR gene. This RFLP was used as a marker in a genetic cosegregation study to examine the correlation of genotype with blood pressure in F2 rats of SHR and WKY rat cross. The distribution of the genotypes based on the RFLP conformed to a 1:2:1 ratio in male F2 rats as expected for Mendelian inheritance, but not in female F2 rats. The number of female F2 rats with double SHR alleles was decreased about 68% from expected. Blood pressure of the male or female F2 rats homozygous for the SHR α2-adrenoceptor alleles was not significantly higher than the F2 rats heterozygous or homozygous for the WKY alleles with either indirect or direct blood pressure measurement. The additional finding was that males had higher blood pressure than females. This study ruled out the cosegregation of α2-adrenoceptor RFLP with higher blood pressure in the F2 rats of SHR and WKY cross. However, Since the abnormality of the renal α2-adrenoceptor in genetically hypertensive rats is one of altered regulation, and the RFLP we found is located in the 5′ region of the gene where functionally important sequences are located which regulate gene expression, this RFLP may still be of interest in genetically hypertensive rats. (Hypertens Res 1992; 15: 143-148).

KW - RFLP

KW - SHR

KW - α-adrenoceptor

UR - http://www.scopus.com/inward/record.url?scp=85008091934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008091934&partnerID=8YFLogxK

U2 - 10.1291/hypres.15.143

DO - 10.1291/hypres.15.143

M3 - Article

AN - SCOPUS:85008091934

VL - 15

SP - 143

EP - 148

JO - Hypertension Research

JF - Hypertension Research

SN - 0916-9636

ER -