Mapping of avirulence genes in the rice blast fungus, Magnaporthe grisea, with RFLP and RAPD markers

Waly Dioh, Didier Tharreau, Jean Loup Notteghem, Marc Joel Orbach, Marc Henri Lebrun

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Three genetically independent avirulence genes, AVR1-1rat7, AVR1-MedNoi, and AVR1-Ku86, were identified in a cross involving isolates Guy11 and 2/0/3 of the rice blast fungus, Magnaporthe grisea. Using 76 random progeny, we constructed a partial genetic map with restriction fragment length polymorphism (RFLP) markers revealed by probes such as the repeated sequences MGL/MGR583 and Pot3/MGR586, cosmids from the M. grisea genetic map, and a telomere sequence oligonucleotide. Avirulence genes AVR1-MedNoi and AVR1-Ku86 were closely linked to telomere RFLPs such as marker TelG (6 cM from AVR1-MedNoi) and TelF (4.5 cM from AVR1-Ku86). Avirulence gene AVR1-Irat7 was linked to a cosmid RFLP located on chromosome 1 and mapped at 20 cM from the avirulence gene AVR1-C039. Using hulked segregant analysis, we identified 11 random amplified polymorphic DNA (RAPD) markers closely linked (0 to 10 cM) to the avirulence genes segregating in this cross. Most of these RAPD markers corresponded to junction fragments between known or new transposons and a single-copy sequence. Such junctions or the whole sequences of single-copy RAPD markers were frequently absent in one parental isolate. Single-copy sequences from RAPD markers tightly linked to avirulence genes will be used for positional cloning.

Original languageEnglish (US)
Pages (from-to)217-227
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume13
Issue number2
StatePublished - Feb 2000

Fingerprint

Magnaporthe
random amplified polymorphic DNA technique
Magnaporthe grisea
blast disease
Chromosome Mapping
Fungi
Polymorphism
Genetic Markers
Restriction Fragment Length Polymorphisms
chromosome mapping
restriction fragment length polymorphism
Genes
fungi
genetic markers
DNA
Cosmids
genes
telomeres
Telomere
Cloning

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Mapping of avirulence genes in the rice blast fungus, Magnaporthe grisea, with RFLP and RAPD markers. / Dioh, Waly; Tharreau, Didier; Notteghem, Jean Loup; Orbach, Marc Joel; Lebrun, Marc Henri.

In: Molecular Plant-Microbe Interactions, Vol. 13, No. 2, 02.2000, p. 217-227.

Research output: Contribution to journalArticle

Dioh, Waly ; Tharreau, Didier ; Notteghem, Jean Loup ; Orbach, Marc Joel ; Lebrun, Marc Henri. / Mapping of avirulence genes in the rice blast fungus, Magnaporthe grisea, with RFLP and RAPD markers. In: Molecular Plant-Microbe Interactions. 2000 ; Vol. 13, No. 2. pp. 217-227.
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