Mapping the intracytoplasmic regions of the α granulocyte-macrophage colony-stimulating factor receptor necessary for cell growth regulation

Alla Polotskaya, Yanming Zhao, Michael B. Lilly, Andrew Kraft

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor is composed of an α subunit which binds GM-CSF and a β subunit, which together form the high affinity receptor. By transfecting the human α subunit into murine Ba/F3 cells, we have been able to investigate the role of the short 54-amino acid intracytoplasmic portion (amino acid 346-400) of this subunit in mediating cell growth. We have shown that the intracytoplasmic amino acids 346-382 are necessary for GM-CSF-mediated cell growth. In contrast, amino acids 382-400 can be removed without effect. The stable transfection of the human β subunit into the cell lines containing the mutant α subunits did not affect the growth characteristics of these cells. The ability of GM-CSF to stimulate cell growth of the Ba/F3 cells α subunit transfectants was correlated with the ability of this hormone to translocate protein kinase C to the particulate fraction. In contrast, the ability of GM-CSF addition to increase phosphorylation of the human β subunit did not correlate with cell growth and required the entire intracytoplasmic domain of the α subunit. These results demonstrate an important role for the intracytoplasmic portion of the α subunit in mediating both signal transduction and cell cycle commitment stimulated by GM-CSF.

Original languageEnglish (US)
Pages (from-to)14607-14613
Number of pages7
JournalJournal of Biological Chemistry
Volume269
Issue number20
StatePublished - May 20 1994
Externally publishedYes

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Granulocyte-Macrophage Colony-Stimulating Factor Receptors
Cell growth
Granulocyte-Macrophage Colony-Stimulating Factor
Amino Acids
Growth
Cells
Signal transduction
Phosphorylation
Protein Kinase C
Hormones
Transfection
Signal Transduction
Cell Cycle
Cell Line

ASJC Scopus subject areas

  • Biochemistry

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Mapping the intracytoplasmic regions of the α granulocyte-macrophage colony-stimulating factor receptor necessary for cell growth regulation. / Polotskaya, Alla; Zhao, Yanming; Lilly, Michael B.; Kraft, Andrew.

In: Journal of Biological Chemistry, Vol. 269, No. 20, 20.05.1994, p. 14607-14613.

Research output: Contribution to journalArticle

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