Mapping the micromechanical properties of cryo-sectioned aortic tissue with Scanning Acoustic Microscopy

Riaz Akhtar, Michael J. Sherratt, Rachel E B Watson, Tribikram Kundu, Brian Derby

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Although the gross mechanical properties of ageing tissues have been extensively documented, biological tissues are highly heterogeneous and little is known concerning the variation of micro-mechanical properties within tissues. Here, we use Scanning Acoustic Microscopy (SAM) to map the acoustic wave speed (a measure of stiffness) as a function of distance from the outer adventitial layer of cryo-sectioned ferret aorta. With a 400 MHz lens, the images of the aorta samples matched those obtained following chemical fixation and staining of sections which were viewed with fluorescence microscopy. Quantitative analysis was conducted with a frequency scanning or V(f) technique by imaging the tissue from 960 MHz to 1.1 GHz. Undulating acoustic wave speed (stiffness) distributions corresponded with elastic fibre locations in the tissue; there was a decrease in wave speed of around 40 ms -1 from the adventitia (outer layer) to the intima (innermost).

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium Proceedings
Pages66-71
Number of pages6
Volume1146
StatePublished - 2008
Event2008 MRS Fall Meeting - Boston, MA, United States
Duration: Dec 1 2008Dec 5 2008

Other

Other2008 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period12/1/0812/5/08

Fingerprint

Tissue
microscopy
scanning
acoustics
aorta
stiffness
Stiffness
Acoustic waves
mechanical properties
frequency scanning
Mechanical properties
Fluorescence microscopy
staining
quantitative analysis
Lenses
Aging of materials
lenses
Acoustic Microscopy
Scanning
Imaging techniques

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Akhtar, R., Sherratt, M. J., Watson, R. E. B., Kundu, T., & Derby, B. (2008). Mapping the micromechanical properties of cryo-sectioned aortic tissue with Scanning Acoustic Microscopy. In Materials Research Society Symposium Proceedings (Vol. 1146, pp. 66-71)

Mapping the micromechanical properties of cryo-sectioned aortic tissue with Scanning Acoustic Microscopy. / Akhtar, Riaz; Sherratt, Michael J.; Watson, Rachel E B; Kundu, Tribikram; Derby, Brian.

Materials Research Society Symposium Proceedings. Vol. 1146 2008. p. 66-71.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Akhtar, R, Sherratt, MJ, Watson, REB, Kundu, T & Derby, B 2008, Mapping the micromechanical properties of cryo-sectioned aortic tissue with Scanning Acoustic Microscopy. in Materials Research Society Symposium Proceedings. vol. 1146, pp. 66-71, 2008 MRS Fall Meeting, Boston, MA, United States, 12/1/08.
Akhtar R, Sherratt MJ, Watson REB, Kundu T, Derby B. Mapping the micromechanical properties of cryo-sectioned aortic tissue with Scanning Acoustic Microscopy. In Materials Research Society Symposium Proceedings. Vol. 1146. 2008. p. 66-71
Akhtar, Riaz ; Sherratt, Michael J. ; Watson, Rachel E B ; Kundu, Tribikram ; Derby, Brian. / Mapping the micromechanical properties of cryo-sectioned aortic tissue with Scanning Acoustic Microscopy. Materials Research Society Symposium Proceedings. Vol. 1146 2008. pp. 66-71
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