Marker assisted genetic analysis of non-brittle rachis trait in barley

Nejdet Kandemir, A. Yildirim, David A Kudrna, P. M. Hayes, A. Kleinhofs

Research output: Contribution to journalArticle

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Abstract

Brittle rachis is a head shattering mechanism of barley. Two tightly linked complementary genes, btr1 and btr2, were believed to control the non-brittle rachis trait. Position of non-brittle rachis loci btr1btr2 on the short arm of Chromosome 3 was investigated using RFLP markers. Two approaches were employed. First, a Hordeum vulgare subsp. spontaneum fragment that confers brittleness in a cv. Bowman near isogenic line was detected. This fragment is 18-33 cM in length and contains MWG798B, ABG057, MWG014, BCD706 and KFP216 markers of the short arm of Chromosome 3. In the second approach, position of btr1 locus in a H. vulgare subsp. spontaneum (Wadi Qilt 23-38) × H. vulgare subsp. vulgare (cv. Harrington) cross was detected using a selective genotyping approach in BC2F1 generation. F-tests and analysis of genotypic compositions of BC2F1 lines showed that btr1 locus, and supposedly the tightly linked btr2 locus, is in 4.3 cM KFP216-RisP114 interval of short arm of Chromosome 3. Results also yielded clues for the presence of at least two additional loci that affect the non-brittle rachis trait. Allelism, tests using genotypes with known non-brittle rachis gene compositions provided additional evidence for presence of such loci.

Original languageEnglish (US)
Pages (from-to)272-277
Number of pages6
JournalHereditas
Volume141
Issue number3
DOIs
StatePublished - 2004

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Hordeum
Genetic Markers
Chromosomes, Human, Pair 3
Genetic Complementation Test
Restriction Fragment Length Polymorphisms
Genes
Head
Genotype

ASJC Scopus subject areas

  • Genetics

Cite this

Marker assisted genetic analysis of non-brittle rachis trait in barley. / Kandemir, Nejdet; Yildirim, A.; Kudrna, David A; Hayes, P. M.; Kleinhofs, A.

In: Hereditas, Vol. 141, No. 3, 2004, p. 272-277.

Research output: Contribution to journalArticle

Kandemir, N, Yildirim, A, Kudrna, DA, Hayes, PM & Kleinhofs, A 2004, 'Marker assisted genetic analysis of non-brittle rachis trait in barley', Hereditas, vol. 141, no. 3, pp. 272-277. https://doi.org/10.1111/j.1601-5223.2004.01841.x
Kandemir, Nejdet ; Yildirim, A. ; Kudrna, David A ; Hayes, P. M. ; Kleinhofs, A. / Marker assisted genetic analysis of non-brittle rachis trait in barley. In: Hereditas. 2004 ; Vol. 141, No. 3. pp. 272-277.
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