Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels

Peng Chen, Ulrike Werner-Zwansiger, Donald Wiesler, Marty Pagel, Milos V. Novotny

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Perbenzoylated sialooligosaccharides were found to be stable derivatives, giving intense signals during the matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis in the positive-ion mode. Terminal Neu5NAcα2→3 and α2→6Gal units of oligosaccharides undergo characteristic structural changes during benzoylation, yielding easily recognizable mass spectral patterns. Subpicomole carbohydrate samples were successfully benzoylated and analyzed through MALDI mass spectrometry.

Original languageEnglish (US)
Pages (from-to)4969-4973
Number of pages5
JournalAnalytical chemistry
Volume71
Issue number21
DOIs
StatePublished - Nov 1 1999

ASJC Scopus subject areas

  • Analytical Chemistry

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