Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels

Peng Chen; Ulrike Werner-Zwansiger; Donald Wiesler; Marty Pagel; Milos V. Novotny

doi:10.1021/ac990674w

Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels

Peng Chen, Ulrike Werner-Zwansiger, Donald Wiesler, Marty Pagel, Milos V. Novotny

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Perbenzoylated sialooligosaccharides were found to be stable derivatives, giving intense signals during the matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis in the positive-ion mode. Terminal Neu5NAcα2→3 and α2→6Gal units of oligosaccharides undergo characteristic structural changes during benzoylation, yielding easily recognizable mass spectral patterns. Subpicomole carbohydrate samples were successfully benzoylated and analyzed through MALDI mass spectrometry.

Original language	English (US)
Pages (from-to)	4969-4973
Number of pages	5
Journal	Analytical chemistry
Volume	71
Issue number	21
DOIs	https://doi.org/10.1021/ac990674w
State	Published - Nov 1 1999

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac990674w

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Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels. / Chen, Peng; Werner-Zwansiger, Ulrike; Wiesler, Donald; Pagel, Marty; Novotny, Milos V.

In: Analytical chemistry, Vol. 71, No. 21, 01.11.1999, p. 4969-4973.

Research output: Contribution to journal › Article › peer-review

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AU - Novotny, Milos V.

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Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels

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