Mass spectrometric imaging of peptide release from neuronal cells within microfluidic devices

Kyubong Jo, Michael L. Heien, Lucas B. Thompson, Ming Zhong, Ralph G. Nuzzo, Jonathan V. Sweedler

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Microfluidic devices are well suited for manipulating and measuring mass limited samples. Here we adapt a microfluidic device containing functionalized surfaces to chemically stimulate a small number of neurons (down to a single neuron), collect the release of neuropeptides, and characterize them using mass spectrometry. As only a small fraction of the peptides present in a neuron are released with physiologically relevant stimulations, the amount of material available for measurement is small, thereby requiring minimal sample loss and high-sensitivity detection. Although a number of detection schemes are used with microfluidic devices, mass spectrometric detection is used here because of its high information content, allowing the characterization of the released peptide complement. Rather than using an on-line approach, off-line analysis is used; after collection of the peptides onto a surface, mass spectrometric imaging interrogates that surface to determine the peptides released from the cell. The overall utility of this scheme is demonstrated using several device formats with measurement of neuropeptides released from Aplysia californica bag cell neurons.

Original languageEnglish (US)
Pages (from-to)1454-1460
Number of pages7
JournalLab on a Chip
Volume7
Issue number11
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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