Mass spectrometry imaging of mating Tetrahymena show that changes in cell morphology regulate lipid domain formation

Michael E. Kurczy, Paul D. Piehowski, Craig T. Van Bell, Michael L Heien, Nicolas Winograd, Andrew G. Ewing

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Mass spectrometry imaging has been used here to suggest that changes in membrane structure drive lipid domain formation in mating single-cell organisms. Chemical studies of lipid bilayers in both living and model systems have revealed that chemical composition is coupled to localized membrane structure. However, it is not clear if the lipids that compose the membrane actively modify membrane structure or if structural changes cause heterogeneity in the surface chemistry of the lipid bilayer. We report that time-of-flight secondary ion mass spectrometry images of mating Tetrahymena thermophila acquired at various stages during mating demonstrate that lipid domain formation, identified as a decrease in the lamellar lipid phosphatidylcholine, follows rather than precedes structural changes in the membrane. Domains are formed in response to structural changes that occur during cell-to-cell conjugation. This observation has wide implications in all membrane processes.

Original languageEnglish (US)
Pages (from-to)2751-2756
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number7
DOIs
StatePublished - Feb 16 2010
Externally publishedYes

Fingerprint

Tetrahymena
Mass Spectrometry
Lipids
Membranes
Lipid Bilayers
Tetrahymena thermophila
Secondary Ion Mass Spectrometry
Membrane Lipids
Phosphatidylcholines

Keywords

  • Lipid domains
  • Membranes
  • Phosphatidylcholine

ASJC Scopus subject areas

  • General

Cite this

Mass spectrometry imaging of mating Tetrahymena show that changes in cell morphology regulate lipid domain formation. / Kurczy, Michael E.; Piehowski, Paul D.; Van Bell, Craig T.; Heien, Michael L; Winograd, Nicolas; Ewing, Andrew G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 7, 16.02.2010, p. 2751-2756.

Research output: Contribution to journalArticle

Kurczy, Michael E. ; Piehowski, Paul D. ; Van Bell, Craig T. ; Heien, Michael L ; Winograd, Nicolas ; Ewing, Andrew G. / Mass spectrometry imaging of mating Tetrahymena show that changes in cell morphology regulate lipid domain formation. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 7. pp. 2751-2756.
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