A sensitive and specific method for the determination of chlorambucil and its metabolite in biological fluids is reported. The method is based on selected‐ion monitoring detection following simple extraction of the parent compound, its metabolite, and an internal standard (chlorambucil‐d8) from plasma and urine samples. The precision (reproducibility) of the method was 94.3 ± 1.3% with 200 ng of chlorambucil added to 1 ml of plasma. Chlorambucil degradation or alkylation of plasma proteins was minimal with plasma incubated at 24° for 4 hr. However, chloroambucil recovery decreased to 56% after plasma incubation at 37° for 4 hr. Three chlorambucil degradation products in ethyl acetate solution were found, and their structures were studied by mass spectrometry.
- Antineoplastic agents—chlorambucil, degradation products and metabolite, mass spectrometry, human plasma and urine
- Chlorambucil—analysis, mass spectrometry, degradation products and metabolite, human plasma and urine
- Mass spectrometry, selected‐ion monitoring—chlorambucil, degradation products and metabolite, human plasma and urine
ASJC Scopus subject areas
- Pharmaceutical Science