Mass spectrometry of chlorambucil, its degradation products, and its metabolite in biological samples

S. Y. Chang, B. J. Larcom, David S Alberts, B. Larsen, P. D. Walson, I. G. Sipes

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A sensitive and specific method for the determination of chlorambucil and its metabolite in biological fluids is reported. The method is based on selected-ion monitoring detection following simple extraction of the parent compound, its metabolite, and an internal standard (chlorambucil-d 8) from plasma and urine samples. The precision (reproducibility) of the method was 94.3 ± 1.3% with 200 ng of chlorambucil added to 1 ml of plasma. Chlorambucil degradation or alkylation of plasma proteins was minimal with plasma incubated at 24° for 4 hr. However, chlorambucil recovery decreased to 56% after plasma incubation at 37° for 4 hr. Three chlorambucil degradation products in ethyl acetate solution were found, and their structures were studied by mass spectrometry.

Original languageEnglish (US)
Pages (from-to)80-84
Number of pages5
JournalJournal of Pharmaceutical Sciences
Volume69
Issue number1
StatePublished - 1980

Fingerprint

Chlorambucil
Metabolites
Mass spectrometry
Mass Spectrometry
Degradation
Plasmas
Alkylation
Blood Proteins
Urine
Ions
Recovery
Fluids
Monitoring

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science

Cite this

Mass spectrometry of chlorambucil, its degradation products, and its metabolite in biological samples. / Chang, S. Y.; Larcom, B. J.; Alberts, David S; Larsen, B.; Walson, P. D.; Sipes, I. G.

In: Journal of Pharmaceutical Sciences, Vol. 69, No. 1, 1980, p. 80-84.

Research output: Contribution to journalArticle

Chang, S. Y. ; Larcom, B. J. ; Alberts, David S ; Larsen, B. ; Walson, P. D. ; Sipes, I. G. / Mass spectrometry of chlorambucil, its degradation products, and its metabolite in biological samples. In: Journal of Pharmaceutical Sciences. 1980 ; Vol. 69, No. 1. pp. 80-84.
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