Mass spectrometry of chlorambucil, its degradation products, and its metabolite in biological samples

S. Y. Chang, B. J. Larcom, D. S. Alberts, B. Larsen, P. D. Walson, I. G. Sipes

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A sensitive and specific method for the determination of chlorambucil and its metabolite in biological fluids is reported. The method is based on selected‐ion monitoring detection following simple extraction of the parent compound, its metabolite, and an internal standard (chlorambucil‐d8) from plasma and urine samples. The precision (reproducibility) of the method was 94.3 ± 1.3% with 200 ng of chlorambucil added to 1 ml of plasma. Chlorambucil degradation or alkylation of plasma proteins was minimal with plasma incubated at 24° for 4 hr. However, chloroambucil recovery decreased to 56% after plasma incubation at 37° for 4 hr. Three chlorambucil degradation products in ethyl acetate solution were found, and their structures were studied by mass spectrometry.

Original languageEnglish (US)
Pages (from-to)80-84
Number of pages5
JournalJournal of pharmaceutical sciences
Volume69
Issue number1
DOIs
StatePublished - Jan 1980

Keywords

  • Antineoplastic agents—chlorambucil, degradation products and metabolite, mass spectrometry, human plasma and urine
  • Chlorambucil—analysis, mass spectrometry, degradation products and metabolite, human plasma and urine
  • Mass spectrometry, selected‐ion monitoring—chlorambucil, degradation products and metabolite, human plasma and urine

ASJC Scopus subject areas

  • Pharmaceutical Science

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