Maternal components of RNA-directed DNA methylation are required for seed development in Brassica rapa

Jeffrey W. Grover, Timmy Kendall, Abdul Baten, Diane Burgess, Michael Freeling, Graham J. King, Rebecca Ann Mosher Harris

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Small RNAs trigger repressive DNA methylation at thousands of transposable elements in a process called RNA-directed DNA methylation (RdDM). The molecular mechanism of RdDM is well characterized in Arabidopsis, yet the biological function remains unclear, as loss of RdDM in Arabidopsis causes no overt defects, even after generations of inbreeding. It is known that 24 nucleotide Pol IV-dependent siRNAs, the hallmark of RdDM, are abundant in flowers and developing seeds, indicating that RdDM might be important during reproduction. Here we show that, unlike Arabidopsis, mutations in the Pol IV-dependent small RNA pathway cause severe and specific reproductive defects in Brassica rapa. High rates of abortion occur when seeds have RdDM mutant mothers, but not when they have mutant fathers. Although abortion occurs after fertilization, RdDM function is required in maternal somatic tissue, not in the female gametophyte or the developing zygote, suggesting that siRNAs from the maternal soma might function in filial tissues. We propose that recently outbreeding species such as B. rapa are key to understanding the role of RdDM during plant reproduction.

Original languageEnglish (US)
Pages (from-to)575-582
Number of pages8
JournalPlant Journal
Volume94
Issue number4
DOIs
StatePublished - May 1 2018

Fingerprint

Brassica rapa
DNA methylation
DNA Methylation
seed development
Seeds
Mothers
RNA
Arabidopsis
abortion (plants)
Reproduction
outbreeding
mutants
plant reproduction
DNA Transposable Elements
Inbreeding
Induced Abortion
Zygote
Carisoprodol
fathers
zygote

Keywords

  • Brassica rapa
  • polymerase IV
  • polymerase V
  • RNA-directed DNA methylation
  • seed development
  • siRNA

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Maternal components of RNA-directed DNA methylation are required for seed development in Brassica rapa. / Grover, Jeffrey W.; Kendall, Timmy; Baten, Abdul; Burgess, Diane; Freeling, Michael; King, Graham J.; Harris, Rebecca Ann Mosher.

In: Plant Journal, Vol. 94, No. 4, 01.05.2018, p. 575-582.

Research output: Contribution to journalArticle

Grover, JW, Kendall, T, Baten, A, Burgess, D, Freeling, M, King, GJ & Harris, RAM 2018, 'Maternal components of RNA-directed DNA methylation are required for seed development in Brassica rapa', Plant Journal, vol. 94, no. 4, pp. 575-582. https://doi.org/10.1111/tpj.13910
Grover JW, Kendall T, Baten A, Burgess D, Freeling M, King GJ et al. Maternal components of RNA-directed DNA methylation are required for seed development in Brassica rapa. Plant Journal. 2018 May 1;94(4):575-582. https://doi.org/10.1111/tpj.13910
Grover, Jeffrey W. ; Kendall, Timmy ; Baten, Abdul ; Burgess, Diane ; Freeling, Michael ; King, Graham J. ; Harris, Rebecca Ann Mosher. / Maternal components of RNA-directed DNA methylation are required for seed development in Brassica rapa. In: Plant Journal. 2018 ; Vol. 94, No. 4. pp. 575-582.
@article{0e91ab9a8f6b45b4a8f23e23a7912265,
title = "Maternal components of RNA-directed DNA methylation are required for seed development in Brassica rapa",
abstract = "Small RNAs trigger repressive DNA methylation at thousands of transposable elements in a process called RNA-directed DNA methylation (RdDM). The molecular mechanism of RdDM is well characterized in Arabidopsis, yet the biological function remains unclear, as loss of RdDM in Arabidopsis causes no overt defects, even after generations of inbreeding. It is known that 24 nucleotide Pol IV-dependent siRNAs, the hallmark of RdDM, are abundant in flowers and developing seeds, indicating that RdDM might be important during reproduction. Here we show that, unlike Arabidopsis, mutations in the Pol IV-dependent small RNA pathway cause severe and specific reproductive defects in Brassica rapa. High rates of abortion occur when seeds have RdDM mutant mothers, but not when they have mutant fathers. Although abortion occurs after fertilization, RdDM function is required in maternal somatic tissue, not in the female gametophyte or the developing zygote, suggesting that siRNAs from the maternal soma might function in filial tissues. We propose that recently outbreeding species such as B. rapa are key to understanding the role of RdDM during plant reproduction.",
keywords = "Brassica rapa, polymerase IV, polymerase V, RNA-directed DNA methylation, seed development, siRNA",
author = "Grover, {Jeffrey W.} and Timmy Kendall and Abdul Baten and Diane Burgess and Michael Freeling and King, {Graham J.} and Harris, {Rebecca Ann Mosher}",
year = "2018",
month = "5",
day = "1",
doi = "10.1111/tpj.13910",
language = "English (US)",
volume = "94",
pages = "575--582",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Maternal components of RNA-directed DNA methylation are required for seed development in Brassica rapa

AU - Grover, Jeffrey W.

AU - Kendall, Timmy

AU - Baten, Abdul

AU - Burgess, Diane

AU - Freeling, Michael

AU - King, Graham J.

AU - Harris, Rebecca Ann Mosher

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Small RNAs trigger repressive DNA methylation at thousands of transposable elements in a process called RNA-directed DNA methylation (RdDM). The molecular mechanism of RdDM is well characterized in Arabidopsis, yet the biological function remains unclear, as loss of RdDM in Arabidopsis causes no overt defects, even after generations of inbreeding. It is known that 24 nucleotide Pol IV-dependent siRNAs, the hallmark of RdDM, are abundant in flowers and developing seeds, indicating that RdDM might be important during reproduction. Here we show that, unlike Arabidopsis, mutations in the Pol IV-dependent small RNA pathway cause severe and specific reproductive defects in Brassica rapa. High rates of abortion occur when seeds have RdDM mutant mothers, but not when they have mutant fathers. Although abortion occurs after fertilization, RdDM function is required in maternal somatic tissue, not in the female gametophyte or the developing zygote, suggesting that siRNAs from the maternal soma might function in filial tissues. We propose that recently outbreeding species such as B. rapa are key to understanding the role of RdDM during plant reproduction.

AB - Small RNAs trigger repressive DNA methylation at thousands of transposable elements in a process called RNA-directed DNA methylation (RdDM). The molecular mechanism of RdDM is well characterized in Arabidopsis, yet the biological function remains unclear, as loss of RdDM in Arabidopsis causes no overt defects, even after generations of inbreeding. It is known that 24 nucleotide Pol IV-dependent siRNAs, the hallmark of RdDM, are abundant in flowers and developing seeds, indicating that RdDM might be important during reproduction. Here we show that, unlike Arabidopsis, mutations in the Pol IV-dependent small RNA pathway cause severe and specific reproductive defects in Brassica rapa. High rates of abortion occur when seeds have RdDM mutant mothers, but not when they have mutant fathers. Although abortion occurs after fertilization, RdDM function is required in maternal somatic tissue, not in the female gametophyte or the developing zygote, suggesting that siRNAs from the maternal soma might function in filial tissues. We propose that recently outbreeding species such as B. rapa are key to understanding the role of RdDM during plant reproduction.

KW - Brassica rapa

KW - polymerase IV

KW - polymerase V

KW - RNA-directed DNA methylation

KW - seed development

KW - siRNA

UR - http://www.scopus.com/inward/record.url?scp=85046495794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046495794&partnerID=8YFLogxK

U2 - 10.1111/tpj.13910

DO - 10.1111/tpj.13910

M3 - Article

C2 - 29569777

AN - SCOPUS:85046495794

VL - 94

SP - 575

EP - 582

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 4

ER -