Maternal RNA-directed DNA methylation is required for seed development in Brassica rapa

Jeffrey W. Grover; Timmy Kendall; Abdul Baten; Graham J. King; Rebecca A. Mosher

doi:10.1101/174805

Maternal RNA-directed DNA methylation is required for seed development in Brassica rapa

Jeffrey W. Grover, Timmy Kendall, Abdul Baten, Graham J. King, Rebecca A. Mosher

Plant Sciences, School of

Research output: Contribution to journal › Article › peer-review

Abstract

Some organisms deploy small RNAs from accessory cells to maintain genome integrity in the zygote, a mechanism that has been proposed but not demonstrated in plants. Here we show that maternal mutations in the Pol IV-dependent small RNA pathway cause abortion of developing seeds in Brassica rapa. Surprisingly, small RNA production is required in maternal somatic tissues, but not in maternal gametes or the developing zygote. We propose that parental influence over zygotic genomes is a common strategy in eukaryotes and that outbreeding species such as B. rapa are key to understanding the role of small RNAs during reproduction.

Original language	English (US)
Journal	Unknown Journal
DOIs	https://doi.org/10.1101/174805
State	Published - Aug 10 2017

Keywords

Polymerase IV
Polymerase V
RNA-directed DNA Methylation
Seed development
SiRNA

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
Immunology and Microbiology(all)
Neuroscience(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1101/174805

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title = "Maternal RNA-directed DNA methylation is required for seed development in Brassica rapa",

abstract = "Some organisms deploy small RNAs from accessory cells to maintain genome integrity in the zygote, a mechanism that has been proposed but not demonstrated in plants. Here we show that maternal mutations in the Pol IV-dependent small RNA pathway cause abortion of developing seeds in Brassica rapa. Surprisingly, small RNA production is required in maternal somatic tissues, but not in maternal gametes or the developing zygote. We propose that parental influence over zygotic genomes is a common strategy in eukaryotes and that outbreeding species such as B. rapa are key to understanding the role of small RNAs during reproduction.",

keywords = "Polymerase IV, Polymerase V, RNA-directed DNA Methylation, Seed development, SiRNA",

author = "Grover, {Jeffrey W.} and Timmy Kendall and Abdul Baten and King, {Graham J.} and Mosher, {Rebecca A.}",

note = "Publisher Copyright: The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2017",

month = aug,

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doi = "10.1101/174805",

language = "English (US)",

journal = "Nuclear Physics A",

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AU - Grover, Jeffrey W.

AU - Kendall, Timmy

AU - Baten, Abdul

AU - King, Graham J.

AU - Mosher, Rebecca A.

N1 - Publisher Copyright: The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2017/8/10

Y1 - 2017/8/10

N2 - Some organisms deploy small RNAs from accessory cells to maintain genome integrity in the zygote, a mechanism that has been proposed but not demonstrated in plants. Here we show that maternal mutations in the Pol IV-dependent small RNA pathway cause abortion of developing seeds in Brassica rapa. Surprisingly, small RNA production is required in maternal somatic tissues, but not in maternal gametes or the developing zygote. We propose that parental influence over zygotic genomes is a common strategy in eukaryotes and that outbreeding species such as B. rapa are key to understanding the role of small RNAs during reproduction.

AB - Some organisms deploy small RNAs from accessory cells to maintain genome integrity in the zygote, a mechanism that has been proposed but not demonstrated in plants. Here we show that maternal mutations in the Pol IV-dependent small RNA pathway cause abortion of developing seeds in Brassica rapa. Surprisingly, small RNA production is required in maternal somatic tissues, but not in maternal gametes or the developing zygote. We propose that parental influence over zygotic genomes is a common strategy in eukaryotes and that outbreeding species such as B. rapa are key to understanding the role of small RNAs during reproduction.

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KW - RNA-directed DNA Methylation

KW - Seed development

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