Matrilysin expression in human prostate carcinoma

J. David Knox, Catherine Wolf, Kathleen McDaniel, Virginia Clark, Maria Loriot, G. Tim Bowden, Raymond B Nagle

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The metalloproteinases, a multigene family of metal-requiring enzymes, have been suggested to play a role in tumor invasion and metastasis. Previously, we demonstrated that human primary prostate tumors express higher levels of matrilysin and gelatinase A mRNA than normal prostate does. In the study presented here, we used in situ hybridization and immunohistochemical staining of serial sections of paraffin-embedded primary prostate tumors to compare the sites of matrilysin and gelatinase A expression and protein localization. These results confirmed the epithelial nature of matrilysin expression and protein localization. In contrast, gelatinase A mRNA was localized to the interstitial stroma, whereas the protein was associated with the epithelial tumor cells. In situ hybridization was also used to demonstrate that gelatinase B expression was restricted to macrophages infiltrating the tumors. Proteins isolated from an additional set of frozen tumor specimens were analyzed by western blotting to determine the relative amounts of matrilysin in the active and proenzyme forms. The western analyses demonstrated that in all cases in which matrilysin was detected, at least some of the enzyme was in the active form. These results are discussed with respect to the possible role these enzymes may play in prostate tumor progression.

Original languageEnglish (US)
Pages (from-to)57-63
Number of pages7
JournalMolecular Carcinogenesis
Volume15
Issue number1
DOIs
StatePublished - Jan 1996

Fingerprint

Matrix Metalloproteinase 7
Prostate
Carcinoma
Matrix Metalloproteinase 2
Neoplasms
In Situ Hybridization
Proteins
Enzymes
Messenger RNA
Enzyme Precursors
Matrix Metalloproteinase 9
Metalloproteases
Multigene Family
Paraffin
Western Blotting
Epithelial Cells
Metals
Macrophages
Staining and Labeling
Neoplasm Metastasis

Keywords

  • Gelatinase
  • Immunohistochemistry
  • In situ
  • Metalloproteinase

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Matrilysin expression in human prostate carcinoma. / Knox, J. David; Wolf, Catherine; McDaniel, Kathleen; Clark, Virginia; Loriot, Maria; Bowden, G. Tim; Nagle, Raymond B.

In: Molecular Carcinogenesis, Vol. 15, No. 1, 01.1996, p. 57-63.

Research output: Contribution to journalArticle

Knox, J. David ; Wolf, Catherine ; McDaniel, Kathleen ; Clark, Virginia ; Loriot, Maria ; Bowden, G. Tim ; Nagle, Raymond B. / Matrilysin expression in human prostate carcinoma. In: Molecular Carcinogenesis. 1996 ; Vol. 15, No. 1. pp. 57-63.
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